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Tissue-Specific Stem Cells
Version of Record online: 20 AUG 2012
Copyright © 2012 AlphaMed Press
Volume 30, Issue 9, pages 1971–1984, September 2012
How to Cite
Boldrin, L., Neal, A., Zammit, P. S., Muntoni, F. and Morgan, J. E. (2012), Donor Satellite Cell Engraftment Is SignificantlyAugmented When the Host Niche Is Preserved and Endogenous Satellite Cells Are Incapacitated. STEM CELLS, 30: 1971–1984. doi: 10.1002/stem.1158
Author contributions: L.B.: conception and design, collection and assembly of data, data analysis and interpretation, and manuscript writing; A.N.: collection, assembly, analysis of data presented in Figure 5; P.Z.: intellectual input into the work, critical reading and final approval of the manuscript; F.M.: intellectual input into the work, critical reading and final approval of the manuscript; J.M.: holder of the grants that fund the project, intellectual input into the work, and manuscript writing.
Disclosure of potential conflicts of interest is found at the end of this article.
First published online in STEM CELLSEXPRESS June 21, 2012; available online without subscription through the open access option.
- Issue online: 20 AUG 2012
- Version of Record online: 20 AUG 2012
- Accepted manuscript online: 21 JUN 2012 03:23PM EST
- Manuscript Accepted: 3 JUN 2012
- Manuscript Received: 6 FEB 2012
- Muscular Dystrophy Campaign
- MRC Centre for Neuromuscular Diseases
- Wellcome Trust University award
- Ormond Street Hospital Children's Charity
- Muscular Dystrophy Campaign
- Medical Research Council
- Wellcome Trust. Grant Number: 085137/Z/08/Z
- OPTISTEM. Grant Number: 223098
- BIODESIGN. Grant Number: 262948-2
- European Commission 7th Framework Programme
- Muscular Dystrophy Campaign. Grant Number: RA3 776
- Association Francaise contre les Myopathies. Grant Number: 13948
- Wellcome Trust. Grant Number: 08241/Z/07/Z
Additional Supporting Information may be found in the online version of this article.
|SC_12-0134_sm_supplFigure1.tif||442K||Supplementary Figure 1 Mdx nude mice, whose hind legs were untreated (n=3) or irradiated with 18 Gy (n=5) or 25 Gy (n=5), had both TA muscles removed either on the same day as irradiation (day 0) (n=1, 2 and 3 respectively), or 3 days later (n=1, 2 and 3 respectively). The graph depicts the percentage of CD45+ and CD31+ area found in each case. No significant difference was detected.|
|SC_12-0134_sm_supplFigure2.pdf||103K||Supplementary Figure 2 Self-renewal of donor My5nLacZ/+ satellite cells within host muscles injured in different ways. (a) X-gal staining of whole muscle grafts showed β-gal expressing satellite cells aligned within muscle fibres. Magnifications: 3.2X. (b) Magnified areas highlight donor satellite cell distribution and density along the muscle fibres; donor satellite cells are clearly far more numerous within irradiated host muscles. Magnification: 10X. The incidence of muscles containing donor-derived satellite cells was 5/10 in irradiated, 3/8 in BaCl2-treated, 4/12 in cardiotoxin-treated, 1/8 in notexin-injected muscles and 2/4 in non-treated muscles. We found donor derived satellite cells in ∼36% (15 out of 42) of grafts with no evidence to reject the assumption that different treatments have different efficiencies (Fisher's exact test, p=0.5627).|
|SC_12-0134_sm_supplFigure3.tif||744K||Supplementary Figure 3 Heat map display of data collected from cytokine array on irradiated and non-irradiated mdx nude muscles. Proteins extracted from TA muscles of 3 week old mice, irradiated either 3 days before, or on the same day as, analysis were compared to protein extracted from non-treated TA muscles. Analysis was performed on male and female mice (n=2 in each group).|
|SC_12-0134_sm_supplFigure4.tif||452K||Supplementary Figure 4 Partial refilling of the niche by radiation-resistant satellite cells. 3 week old mdx nude mice had their TA muscles either irradiated (IRR 4weeks) (n=6), or notexin injected immediately after irradiation (IRR+NTX 4weeks) (n=6), or non-treated (n=6) and removed 4 weeks later. The graph shows quantification of satellite cell number per fibre performed on transverse muscle sections immunostained with Pax7 and laminin antibody. Satellite cell number was significantly lower in treated compared to non-treated muscles, but, there were significantly more satellite cells in IRR+NTX 4weeks than in IRR 4weeks TA muscles. (***p<0.001, * p< 0.05).|
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