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Tissue-Specific Stem Cells
Article first published online: 20 AUG 2012
Copyright © 2012 AlphaMed Press
Volume 30, Issue 9, pages 1999–2009, September 2012
How to Cite
Stamp, L. A., Braxton, D. R., Wu, J., Akopian, V., Hasegawa, K., Chandrasoma, P. T., Hawes, S. M., McLean, C., Petrovic, L. M., Wang, K. and Pera, M. F. (2012), The GCTM-5 Epitope Associated with the Mucin-Like Glycoprotein FCGBP Marks Progenitor Cells in Tissues of Endodermal Origin. STEM CELLS, 30: 1999–2009. doi: 10.1002/stem.1167
Author contributions: L.A.S. and D.R.B.: conception and design, collection of data, data analysis and interpretation, manuscript writing, and final approval of manuscript; J.W.: conception and design, collection of data, data analysis and interpretation, and approval of manuscript; V.A.: collection of data; K.H.: conception and design, collection of data, data analysis and interpretation, and final approval of manuscript; P.T.C.: provision of study material and data analysis and interpretation; S.H.: data analysis and interpretation; C.M. and L.M.P.: provision of study material, data analysis and interpretation, and final approval of manuscript; K.W.: financial support, provision of study material, data analysis and interpretation, and final approval of manuscript; M.F.P.: conception and design, financial support, provision of study material, data analysis and interpretation, manuscript writing, and final approval of manuscript. L.A.S., D.R.B., J.W., V.A., and K.H. contributed equally to this article.
Disclosure of potential conflicts of interest is found at the end of this article.
First published online in STEM CELLSEXPRESS July 3, 2012.
- Issue published online: 20 AUG 2012
- Article first published online: 20 AUG 2012
- Accepted manuscript online: 3 JUL 2012 08:02AM EST
- Manuscript Accepted: 25 MAY 2012
- Manuscript Received: 22 MAR 2012
- Juvenile Diabetes Research Foundation
- Australian Stem Cell Centre
- National Institutes of Health (pilot feasibility project of the USC Research Center for Liver Diseases, NIH. Grant Number: P30 DK04852
- California Institute of Regenerative Medicine
- CHLA Stem Cell Training. Grant Number: #: CIRM TG2-01168
Additional Supporting Information may be found in the online version of this article.
|SC_12-0283_sm_supplFigure1.pdf||299K||Supplemental Figure 1. Fetal tissues express the GCTM-5 antigen. (A) GCTM-5 expression, shown in pink, in the crypts of the human fetal gut at 13 weeks gestation, and (B) an antibody isotype negative control. GCTM-5 expression in (C) 16 week and (D) 18.5 week human fetal pancreas. Cytokeratin 19 staining of (E) 16 week and (F) 18.5 week fetal pancreas; detecting a larger number of ductal structures. Scale bar, 200 μm.|
|SC_12-0283_sm_supplFigure2.pdf||189K||Supplemental Figure 2. Distribution of the GCTM-5 antigen in the gastrointestinal tract. Normal gastric epithelia (A) does not react with GCTM-5 however, gastric intestinal metaplasia (B) and gastric carcinoma (C) express the GCTM-5 antigen. (D) The epithelium of the gall bladder stains positive. The normal colonic crypts (E) express GCTM-5 with less intense reactivity in dysplastic colonic lesions (F). Scale bars: A and E, 200 μm; others 500 μm.|
|SC_12-0283_sm_supplFigure3.tif||2468K||Supplemental Figure 3. Non-gastrointestinal definitive endoderm derived tissues express GCTM-5. (A) The ducts of the salivary glands react with GCTM-5. (B) Human paediatric thymus stained with pan-Keratin (red), GCTM-5 (green) reveal GCTM-5/pan-keratin colocalisation in the Hassall's Corpuscles. (C) The GCTM-5 antigen is expressed in the endocervix. Scale bars, 200 μm.|
|SC_12-0283_sm_supplFigure4.tif||784K||Supplemental Figure 4. Some GCTM-5+ cells in biliary atresia livers are also positive for Ki-67. Scale bar, 100um|
|SC_12-0283_sm_supplFigure5.tif||2357K||Supplemental Figure 5. GCTM-5 expression in biliary atresia livers. (A) GCTM-5 expressed on small reactive bile ductules, majority of these cells are CK19+, N-CAM+ & EpCAM+(weak). (B) GCTM-5 stained positive on enlarged bile duct epithelium, majority of these cells are CK19+, N-CAM− & EpCAM+(very weak). (C) GCTM-5 was also found positive on malformed bile duct epithelia, majority of the cells are CK-19+/−, N-CAM−, EpCAM+. (D) GCTM-5 staining was also found on newly formed bile ducts, majority of these cells are CK19+/−, N-CAM− (positive on surrounding cells) & EpCAM+. Scale bar, 100um.|
|SC_12-0283_sm_supplFigure6.pdf||1892K||Supplemental Figure 6. Co-staining of bile duct epithelium in pediatric liver for GCTM-5 and Sox-9.|
|SC_12-0283_sm_supplFigure7.tif||190K||Supplemental Figure 7. Cell surface expression of GCTM-5 on CF-PAC1 pancreatic adenocarcinoma cells. Live CF-PAC1 cells were stained with either isotype control (top panel) or GCTM-5 (bottom panel). Y-axis, cell number; x-axis, fluorescence intensity.|
|SC_12-0283_sm_supplFigure8.tif||452K||Supplemental Figure 8. Detection of transcripts for FCGBP in non-parenchymal cells from normal adult human liver. Non-parenchymal cells were isolated from normal adult liver and fractionated into GCTM-5 positive and negative populations. QRT-PCR was carried out and transcript levels estimated by the delta-delta Ct method relative to housekeeping controls (GAPDH and PPIA) and normal cultured human fibroblast RNA. Means and range of data shown.|
|SC_12-0283_sm_supplTable1.pdf||31K||Supplemental Table 1|
|SC_12-0283_sm_supplTable2.pdf||34K||Supplemental Table 2|
|SC_12-0283_sm_supplTable3.pdf||14K||Supplemental Table 3|
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