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Additional Supporting Information may be found in the online version of this article.

FilenameFormatSizeDescription
SC_12-0283_sm_supplData.pdf10KSupplemental Data
SC_12-0283_sm_supplFigure1.pdf299KSupplemental Figure 1. Fetal tissues express the GCTM-5 antigen. (A) GCTM-5 expression, shown in pink, in the crypts of the human fetal gut at 13 weeks gestation, and (B) an antibody isotype negative control. GCTM-5 expression in (C) 16 week and (D) 18.5 week human fetal pancreas. Cytokeratin 19 staining of (E) 16 week and (F) 18.5 week fetal pancreas; detecting a larger number of ductal structures. Scale bar, 200 μm.
SC_12-0283_sm_supplFigure2.pdf189KSupplemental Figure 2. Distribution of the GCTM-5 antigen in the gastrointestinal tract. Normal gastric epithelia (A) does not react with GCTM-5 however, gastric intestinal metaplasia (B) and gastric carcinoma (C) express the GCTM-5 antigen. (D) The epithelium of the gall bladder stains positive. The normal colonic crypts (E) express GCTM-5 with less intense reactivity in dysplastic colonic lesions (F). Scale bars: A and E, 200 μm; others 500 μm.
SC_12-0283_sm_supplFigure3.tif2468KSupplemental Figure 3. Non-gastrointestinal definitive endoderm derived tissues express GCTM-5. (A) The ducts of the salivary glands react with GCTM-5. (B) Human paediatric thymus stained with pan-Keratin (red), GCTM-5 (green) reveal GCTM-5/pan-keratin colocalisation in the Hassall's Corpuscles. (C) The GCTM-5 antigen is expressed in the endocervix. Scale bars, 200 μm.
SC_12-0283_sm_supplFigure4.tif784KSupplemental Figure 4. Some GCTM-5+ cells in biliary atresia livers are also positive for Ki-67. Scale bar, 100um
SC_12-0283_sm_supplFigure5.tif2357KSupplemental Figure 5. GCTM-5 expression in biliary atresia livers. (A) GCTM-5 expressed on small reactive bile ductules, majority of these cells are CK19+, N-CAM+ & EpCAM+(weak). (B) GCTM-5 stained positive on enlarged bile duct epithelium, majority of these cells are CK19+, N-CAM− & EpCAM+(very weak). (C) GCTM-5 was also found positive on malformed bile duct epithelia, majority of the cells are CK-19+/−, N-CAM−, EpCAM+. (D) GCTM-5 staining was also found on newly formed bile ducts, majority of these cells are CK19+/−, N-CAM− (positive on surrounding cells) & EpCAM+. Scale bar, 100um.
SC_12-0283_sm_supplFigure6.pdf1892KSupplemental Figure 6. Co-staining of bile duct epithelium in pediatric liver for GCTM-5 and Sox-9.
SC_12-0283_sm_supplFigure7.tif190KSupplemental Figure 7. Cell surface expression of GCTM-5 on CF-PAC1 pancreatic adenocarcinoma cells. Live CF-PAC1 cells were stained with either isotype control (top panel) or GCTM-5 (bottom panel). Y-axis, cell number; x-axis, fluorescence intensity.
SC_12-0283_sm_supplFigure8.tif452KSupplemental Figure 8. Detection of transcripts for FCGBP in non-parenchymal cells from normal adult human liver. Non-parenchymal cells were isolated from normal adult liver and fractionated into GCTM-5 positive and negative populations. QRT-PCR was carried out and transcript levels estimated by the delta-delta Ct method relative to housekeeping controls (GAPDH and PPIA) and normal cultured human fibroblast RNA. Means and range of data shown.
SC_12-0283_sm_supplTable1.pdf31KSupplemental Table 1
SC_12-0283_sm_supplTable2.pdf34KSupplemental Table 2
SC_12-0283_sm_supplTable3.pdf14KSupplemental Table 3

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