Additional Supporting Information may be found in the online version of this article.

SC_12-0283_sm_supplData.pdf10KSupplemental Data
SC_12-0283_sm_supplFigure1.pdf299KSupplemental Figure 1. Fetal tissues express the GCTM-5 antigen. (A) GCTM-5 expression, shown in pink, in the crypts of the human fetal gut at 13 weeks gestation, and (B) an antibody isotype negative control. GCTM-5 expression in (C) 16 week and (D) 18.5 week human fetal pancreas. Cytokeratin 19 staining of (E) 16 week and (F) 18.5 week fetal pancreas; detecting a larger number of ductal structures. Scale bar, 200 μm.
SC_12-0283_sm_supplFigure2.pdf189KSupplemental Figure 2. Distribution of the GCTM-5 antigen in the gastrointestinal tract. Normal gastric epithelia (A) does not react with GCTM-5 however, gastric intestinal metaplasia (B) and gastric carcinoma (C) express the GCTM-5 antigen. (D) The epithelium of the gall bladder stains positive. The normal colonic crypts (E) express GCTM-5 with less intense reactivity in dysplastic colonic lesions (F). Scale bars: A and E, 200 μm; others 500 μm.
SC_12-0283_sm_supplFigure3.tif2468KSupplemental Figure 3. Non-gastrointestinal definitive endoderm derived tissues express GCTM-5. (A) The ducts of the salivary glands react with GCTM-5. (B) Human paediatric thymus stained with pan-Keratin (red), GCTM-5 (green) reveal GCTM-5/pan-keratin colocalisation in the Hassall's Corpuscles. (C) The GCTM-5 antigen is expressed in the endocervix. Scale bars, 200 μm.
SC_12-0283_sm_supplFigure4.tif784KSupplemental Figure 4. Some GCTM-5+ cells in biliary atresia livers are also positive for Ki-67. Scale bar, 100um
SC_12-0283_sm_supplFigure5.tif2357KSupplemental Figure 5. GCTM-5 expression in biliary atresia livers. (A) GCTM-5 expressed on small reactive bile ductules, majority of these cells are CK19+, N-CAM+ & EpCAM+(weak). (B) GCTM-5 stained positive on enlarged bile duct epithelium, majority of these cells are CK19+, N-CAM− & EpCAM+(very weak). (C) GCTM-5 was also found positive on malformed bile duct epithelia, majority of the cells are CK-19+/−, N-CAM−, EpCAM+. (D) GCTM-5 staining was also found on newly formed bile ducts, majority of these cells are CK19+/−, N-CAM− (positive on surrounding cells) & EpCAM+. Scale bar, 100um.
SC_12-0283_sm_supplFigure6.pdf1892KSupplemental Figure 6. Co-staining of bile duct epithelium in pediatric liver for GCTM-5 and Sox-9.
SC_12-0283_sm_supplFigure7.tif190KSupplemental Figure 7. Cell surface expression of GCTM-5 on CF-PAC1 pancreatic adenocarcinoma cells. Live CF-PAC1 cells were stained with either isotype control (top panel) or GCTM-5 (bottom panel). Y-axis, cell number; x-axis, fluorescence intensity.
SC_12-0283_sm_supplFigure8.tif452KSupplemental Figure 8. Detection of transcripts for FCGBP in non-parenchymal cells from normal adult human liver. Non-parenchymal cells were isolated from normal adult liver and fractionated into GCTM-5 positive and negative populations. QRT-PCR was carried out and transcript levels estimated by the delta-delta Ct method relative to housekeeping controls (GAPDH and PPIA) and normal cultured human fibroblast RNA. Means and range of data shown.
SC_12-0283_sm_supplTable1.pdf31KSupplemental Table 1
SC_12-0283_sm_supplTable2.pdf34KSupplemental Table 2
SC_12-0283_sm_supplTable3.pdf14KSupplemental Table 3

Please note: Wiley Blackwell is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.