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Stem Cell Technology: Epigenetics, Genomics, Proteomics and Metabonomics
Article first published online: 20 AUG 2012
Copyright © 2012 AlphaMed Press
Volume 30, Issue 9, pages 1800–1807, September 2012
How to Cite
Xia, X. and Wong, S. T. (2012), Concise Review: A High-Content Screening Approach to Stem Cell Research and Drug Discovery. STEM CELLS, 30: 1800–1807. doi: 10.1002/stem.1168
Author contributions: X.X. and S.T.W.: wrote the manuscript.
Disclosure of potential conflicts of interest is found at the end of this article.
First published online in STEM CELLSEXPRESS June July 20, 2012; available online without subscription through the open access option.
- Issue published online: 20 AUG 2012
- Article first published online: 20 AUG 2012
- Accepted manuscript online: 20 JUL 2012 12:01PM EST
- Manuscript Accepted: 16 JUN 2012
- Manuscript Received: 20 APR 2012
- NIH. Grant Number: R01AG028928
- Ting Tsung and Wei Fong Chao Center for Bioinformatics Research and Imaging in Neurosciences (BRAIN)
- Stem cells;
- High-content screening;
- High-throughput screening;
- Drug screening;
- Image-based screening;
- Cell-based assay
High-throughput screening (HTS) is a technology widely used for early stages of drug discovery in pharmaceutical and biotechnology industries. Recent hardware and software improvements have enabled HTS to be used in combination with subcellular resolution microscopy, resulting in cell image-based HTS, called high-content screening (HCS). HCS allows the acquisition of deeper knowledge at a single-cell level such that more complex biological systems can be studied in a high-throughput manner. The technique is particularly well-suited for stem cell research and drug discovery, which almost inevitably require single-cell resolutions for the detection of rare phenotypes in heterogeneous cultures. With growing availability of facilities, instruments, and reagent libraries, small-to-moderate scale HCS can now be carried out in regular academic labs. We envision that the HCS technique will play an increasing role in both basic mechanism study and early-stage drug discovery on stem cells. Here, we review the development of HCS technique and its past application on stem cells and discuss possible future developments. Stem Cells2012;30:1800–1807