Additional Supporting Information may be found in the online version of this article.

SC-12-0131_sm_SupplFigure1.pdf331KSupplementary Figure S1. Wt and ROSA-tomRITVA ES cells show normal chromosomal consistency. (A) and (B): Karyotype of wt 129SvJ parental and ROSAtomRITVA ES cells.
SC-12-0131_sm_SupplFigure2.pdf120KSupplementary Figure S2. ROSA-tomRITVA ES cell-derived neurons show expression of pre- and postsynaptic protein markers. (A): Neurons express Synapsin, (B): Gephyrin, and (C): Bassoon. Scale bars: 60 μm.
SC-12-0131_sm_SupplFigure3.pdf32KSupplementary Figure S3. ROSA-tomRITVA ES cell-derived neurons form synapses with primary cortical neurons. (A) DIC image of synaptic pairs targeted for paired whole cell recordings (scale bar: 10 μm). Presynaptic cell is ROSA-tomRITVA ES cell-derived SADΔG-EGFP infected neuron. Postsynaptic is primary cortical neuron (B): Representative traces of synaptic coupling. Top, presynaptic changes in membrane potential with current injection; bottom, postsynaptic current responses. Responses are blocked via bath application of CNQX, APV, and Bicuculline. Washout rescues synaptic responses (traces are averaged over 15 sweeps of the same neuron).

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