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Embryonic Stem Cells/Induced Pluripotent Stem Cells
Article first published online: 20 SEP 2012
Copyright © 2012 AlphaMed Press
Volume 30, Issue 10, pages 2140–2151, October 2012
How to Cite
Garcia, I., Huang, L., Ung, K. and Arenkiel, B. R. (2012), Tracing Synaptic Connectivity onto Embryonic Stem Cell-derived Neurons. STEM CELLS, 30: 2140–2151. doi: 10.1002/stem.1185
Author contributions: I.G.: experimental design, data collection, and manuscript writing; L.H.: data collection and data analysis; K.U.: data collection; B.A.: experimental conception and design, manuscript writing, and financial support.
Disclosure of potential conflicts of interest is found at the end of this article.
First published online in STEM CELLSEXPRESS July 31, 2012.
- Issue published online: 20 SEP 2012
- Article first published online: 20 SEP 2012
- Accepted manuscript online: 31 JUL 2012 12:38PM EST
- Manuscript Accepted: 22 JUN 2012
- Manuscript Received: 5 FEB 2012
- McNair Medical Institute and NINDS. Grant Numbers: R00NS064171, R01NS078294
Additional Supporting Information may be found in the online version of this article.
|SC-12-0131_sm_SupplFigure1.pdf||331K||Supplementary Figure S1. Wt and ROSA-tomRITVA ES cells show normal chromosomal consistency. (A) and (B): Karyotype of wt 129SvJ parental and ROSAtomRITVA ES cells.|
|SC-12-0131_sm_SupplFigure2.pdf||120K||Supplementary Figure S2. ROSA-tomRITVA ES cell-derived neurons show expression of pre- and postsynaptic protein markers. (A): Neurons express Synapsin, (B): Gephyrin, and (C): Bassoon. Scale bars: 60 μm.|
|SC-12-0131_sm_SupplFigure3.pdf||32K||Supplementary Figure S3. ROSA-tomRITVA ES cell-derived neurons form synapses with primary cortical neurons. (A) DIC image of synaptic pairs targeted for paired whole cell recordings (scale bar: 10 μm). Presynaptic cell is ROSA-tomRITVA ES cell-derived SADΔG-EGFP infected neuron. Postsynaptic is primary cortical neuron (B): Representative traces of synaptic coupling. Top, presynaptic changes in membrane potential with current injection; bottom, postsynaptic current responses. Responses are blocked via bath application of CNQX, APV, and Bicuculline. Washout rescues synaptic responses (traces are averaged over 15 sweeps of the same neuron).|
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