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Embryonic Stem Cells/Induced Pluripotent Stem Cells
Article first published online: 20 SEP 2012
Copyright © 2012 AlphaMed Press
Volume 30, Issue 10, pages 2152–2163, October 2012
How to Cite
Esmailpour, T. and Huang, T. (2012), TBX3 Promotes Human Embryonic Stem Cell Proliferation and Neuroepithelial Differentiation in a Differentiation Stage-dependent Manner. STEM CELLS, 30: 2152–2163. doi: 10.1002/stem.1187
Author contributions: T.E.: conception and design, collection and/or assembly of data, data analysis and interpretation, manuscript writing, and final approval of manuscript; T.H.: conception and design, financial support, provision of study material, data analysis and interpretation, manuscript writing, and final approval of manuscript.
Disclosure of potential conflicts of interest is found at the end of this article.
First published online in STEM CELLSEXPRESS August 2, 2012.
- Issue published online: 20 SEP 2012
- Article first published online: 20 SEP 2012
- Accepted manuscript online: 2 AUG 2012 10:50AM EST
- Manuscript Accepted: 29 JUN 2012
- Manuscript Received: 20 FEB 2012
- National Cancer Institute. Grant Numbers: R01CA121876, R01CA121876
Additional Supporting Information may be found in the online version of this article.
|SC-12-0123_sm_SupplFigure1.tif||1749K||Figure S1. Knockdown of TBX3 does not affect human ESC pluripotency or cell proliferation. (A) Knockdown of TBX3 in human ESCs did not affect colony morphology. (B) Quantitative RT-PCR analysis of pluripotency markers, OCT4 and NANOG. (Control H9 cells =1.0) TBX3 knockdown did not significantly affect OCT4 or NANOG expression. (C) Knockdown of TBX3 did not affect cell proliferation. Mitotic index was calculated as described previously for the over-expression studies. (D) Quantification of neural rosettes in control and TBX3 knockdown cell lines using a defined neuroepithelial differentiation protocol. The loss of TBX3 expression coincided with a significant loss in neural rosette formation.*, p<0.05.|
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