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Tissue-Specific Stem Cells

Dual Lineage-Specific Expression of Sox17 During Mouse Embryogenesis†‡§

  1. Eunyoung Choi1,
  2. Marine R-C. Kraus2,
  3. Laurence A. Lemaire2,3,
  4. Momoko Yoshimoto4,
  5. Sasidhar Vemula4,
  6. Leah A. Potter1,
  7. Elisabetta Manduchi5,
  8. Christian J. Stoeckert Jr.5,
  9. Anne Grapin-Botton2,3,
  10. Mark A. Magnuson1,¶,*

Article first published online: 20 SEP 2012

DOI: 10.1002/stem.1192

Issue

STEM CELLS

STEM CELLS

Volume 30, Issue 10, pages 2297–2308, October 2012

Additional Information

How to Cite

Choi, E., Kraus, M. R.-C., Lemaire, L. A., Yoshimoto, M., Vemula, S., Potter, L. A., Manduchi, E., Stoeckert, C. J., Grapin-Botton, A. and Magnuson, M. A. (2012), Dual Lineage-Specific Expression of Sox17 During Mouse Embryogenesis. STEM CELLS, 30: 2297–2308. doi: 10.1002/stem.1192

Author Information

  1. 1

    Center for Stem Cell Biology and Department of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, Tennessee, USA

  2. 2

    Swiss Institute for Experimental Cancer Research, School of Life Sciences, Ecole Polytechnique Fédérale de Lausanne, Lausanne, Switzerland

  3. 3

    DanStem, University of Copenhagen, Copenhagen N, Denmark

  4. 4

    Department of Pediatrics, Wells Center for Pediatric Research, Indiana University School of Medicine, Indianapolis, Indiana, USA

  5. 5

    Penn Center for Bioinformatics and Department of Genetics, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania, USA

  1. Telephone: 615-322-7006; Fax: 615-332-6645

*9465 MRB-IV, 2213 Garland Avenue, Vanderbilt University School of Medicine, Nashville, Tennessee 37232-0494, USA

  1. †

    Author contributions: E.C.: conception and design, collection and assembly of data, data analysis and interpretation, and manuscript writing; M.R-C.K., L.A.L., M.Y., and S.V.: collection and assembly of data; L.A.P.: data analysis and manuscript writing; E.M. and C.J.S.: data analysis and interpretation and manuscript evaluation; A.G-B.: provision of study material, financial support, and manuscript evaluation; M.A.M: conception and design, data analysis, financial support, manuscript writing, and final approval of manuscript.

  2. ‡

    Disclosure of potential conflicts of interest is found at the end of this article.

  3. §

    First published online in STEM CELLSEXPRESS August 3, 2012. available online without subscription through the open access option

  4. Telephone: 615-322-7006; Fax: 615-332-6645

Publication History

  1. Issue published online: 20 SEP 2012
  2. Article first published online: 20 SEP 2012
  3. Accepted manuscript online: 3 AUG 2012 04:17PM EST
  4. Manuscript Accepted: 17 JUL 2012
  5. Manuscript Received: 13 MAR 2012

Funded by

  • NIH. Grant Numbers: DK72473, DK89523, M.A.M., DK072495, A.G.B.; CA68485, DK58404
  • Vanderbilt Flow Cytometry Shared Resource. Grant Numbers: CA68485, DK20593
  • Vanderbilt Transgenic Mouse/ESC Shared Resource. Grant Number: CA68485
  • Vanderbilt Genome Sciences Resource

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Keywords:

  • Endoderm;
  • Mesoderm;
  • Ventral pancreas;
  • Hemogenic endothelium;
  • Sox17

Abstract

Sox17 is essential for both endoderm development and fetal hematopoietic stem cell (HSC) maintenance. While endoderm-derived organs are well known to originate from Sox17-expressing cells, it is less certain whether fetal HSCs also originate from Sox17-expressing cells. By generating a Sox17GFPCre allele and using it to assess the fate of Sox17-expressing cells during embryogenesis, we confirmed that both endodermal and a part of definitive hematopoietic cells are derived from Sox17-positive cells. Prior to E9.5, the expression of Sox17 is restricted to the endoderm lineage. However, at E9.5 Sox17 is expressed in the endothelial cells (ECs) at the para-aortic splanchnopleural region that contribute to the formation of HSCs at a later stage. The identification of two distinct progenitor cell populations that express Sox17 at E9.5 was confirmed using fluorescence-activated cell sorting together with RNA-Seq to determine the gene expression profiles of the two cell populations. Interestingly, this analysis revealed differences in the RNA processing of the Sox17 mRNA during embryogenesis. Taken together, these results indicate that Sox17 is expressed in progenitor cells derived from two different germ layers, further demonstrating the complex expression pattern of this gene and suggesting caution when using Sox17 as a lineage-specific marker. STEM Cells2012;30:2297–2308

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