Dual Lineage-Specific Expression of Sox17 During Mouse Embryogenesis§

Authors

  • Eunyoung Choi,

    1. Center for Stem Cell Biology and Department of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, Tennessee, USA
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  • Marine R-C. Kraus,

    1. Swiss Institute for Experimental Cancer Research, School of Life Sciences, Ecole Polytechnique Fédérale de Lausanne, Lausanne, Switzerland
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  • Laurence A. Lemaire,

    1. Swiss Institute for Experimental Cancer Research, School of Life Sciences, Ecole Polytechnique Fédérale de Lausanne, Lausanne, Switzerland
    2. DanStem, University of Copenhagen, Copenhagen N, Denmark
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  • Momoko Yoshimoto,

    1. Department of Pediatrics, Wells Center for Pediatric Research, Indiana University School of Medicine, Indianapolis, Indiana, USA
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  • Sasidhar Vemula,

    1. Department of Pediatrics, Wells Center for Pediatric Research, Indiana University School of Medicine, Indianapolis, Indiana, USA
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  • Leah A. Potter,

    1. Center for Stem Cell Biology and Department of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, Tennessee, USA
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  • Elisabetta Manduchi,

    1. Penn Center for Bioinformatics and Department of Genetics, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania, USA
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  • Christian J. Stoeckert Jr.,

    1. Penn Center for Bioinformatics and Department of Genetics, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania, USA
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  • Anne Grapin-Botton,

    1. Swiss Institute for Experimental Cancer Research, School of Life Sciences, Ecole Polytechnique Fédérale de Lausanne, Lausanne, Switzerland
    2. DanStem, University of Copenhagen, Copenhagen N, Denmark
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  • Mark A. Magnuson

    Corresponding author
    1. Center for Stem Cell Biology and Department of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, Tennessee, USA
    • 9465 MRB-IV, 2213 Garland Avenue, Vanderbilt University School of Medicine, Nashville, Tennessee 37232-0494, USA

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    • Telephone: 615-322-7006; Fax: 615-332-6645


  • Author contributions: E.C.: conception and design, collection and assembly of data, data analysis and interpretation, and manuscript writing; M.R-C.K., L.A.L., M.Y., and S.V.: collection and assembly of data; L.A.P.: data analysis and manuscript writing; E.M. and C.J.S.: data analysis and interpretation and manuscript evaluation; A.G-B.: provision of study material, financial support, and manuscript evaluation; M.A.M: conception and design, data analysis, financial support, manuscript writing, and final approval of manuscript.

  • Disclosure of potential conflicts of interest is found at the end of this article.

  • §

    First published online in STEM CELLSEXPRESS August 3, 2012. available online without subscription through the open access option

Abstract

Sox17 is essential for both endoderm development and fetal hematopoietic stem cell (HSC) maintenance. While endoderm-derived organs are well known to originate from Sox17-expressing cells, it is less certain whether fetal HSCs also originate from Sox17-expressing cells. By generating a Sox17GFPCre allele and using it to assess the fate of Sox17-expressing cells during embryogenesis, we confirmed that both endodermal and a part of definitive hematopoietic cells are derived from Sox17-positive cells. Prior to E9.5, the expression of Sox17 is restricted to the endoderm lineage. However, at E9.5 Sox17 is expressed in the endothelial cells (ECs) at the para-aortic splanchnopleural region that contribute to the formation of HSCs at a later stage. The identification of two distinct progenitor cell populations that express Sox17 at E9.5 was confirmed using fluorescence-activated cell sorting together with RNA-Seq to determine the gene expression profiles of the two cell populations. Interestingly, this analysis revealed differences in the RNA processing of the Sox17 mRNA during embryogenesis. Taken together, these results indicate that Sox17 is expressed in progenitor cells derived from two different germ layers, further demonstrating the complex expression pattern of this gene and suggesting caution when using Sox17 as a lineage-specific marker. STEM Cells2012;30:2297–2308

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