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Cancer Stem Cells
Article first published online: 20 SEP 2012
Copyright © 2012 AlphaMed Press
Volume 30, Issue 10, pages 2100–2113, October 2012
How to Cite
Luo, Y., Dallaglio, K., Chen, Y., Robinson, W. A., Robinson, S. E., McCarter, M. D., Wang, J., Gonzalez, R., Thompson, D. C., Norris, D. A., Roop, D. R., Vasiliou, V. and Fujita, M. (2012), ALDH1A Isozymes are Markers of Human Melanoma Stem Cells and Potential Therapeutic Targets. STEM CELLS, 30: 2100–2113. doi: 10.1002/stem.1193
Author contributions: Y.L.: conception and design, collection and assembly of data, data analysis and interpretation, manuscript writing, and final approval of manuscript; K.D., Y.C., and J.W.: collection and assembly of data, data analysis and interpretation, and final approval of manuscript; W.A.R., S.E.R., M.D.M., and R.G.: provision of study material or patients and final approval of manuscript; D.C.T., D.A.N., D.R.R., and V.V.: data analysis and interpretation and final approval of manuscript; M.F.: conception and design, financial support, collection and assembly of data, data analysis and interpretation, manuscript writing, and final approval of manuscript.
Disclosure of potential conflicts of interest is found at the end of this article.
First published online in STEM CELLSEXPRESS August 7, 2012.
- Issue published online: 20 SEP 2012
- Article first published online: 20 SEP 2012
- Accepted manuscript online: 7 AUG 2012 09:00AM EST
- Manuscript Accepted: 26 JUN 2012
- Manuscript Received: 28 FEB 2012
- NIH. Grant Number: CA125833
- Veterans Affairs Merit Review Award
- Wendy Will Case Cancer Fund
- Tadamitsu Cancer Research Fund
- NEI. Grant Numbers: EY17963, EY11490
Additional Supporting Information may be found in the online version of this article.
|sc-12-0203_sm_SupplFigure1.pdf||168K||Figure S1. Expression of ALDH isoforms in human melanoma cell lines. The copy number of human ALDH1A1 (1A1) and ALDH1A3 (1A3) was analyzed by qRT-PCR. mRNAs of ALDH+ and ALDH- subpopulations obtained from human melanoma cell lines (1205Lu, A375, WM239A and HS294T) were analyzed. Copy number was counted and determined by standard curve analysis.|
|sc-12-0203_sm_SupplFigure2.pdf||168K||Figure S2. Representative flow cytometric analysis of GFP-expressing cells. ALDH+1205Lu cells were transfected with GFP-shRNA control plasmid (sh-ctrl) or GFP-shRNA-ALDH1A3 plasmid (sh-1A3). GFP-positive (representing successful transfection) and DAPI-negative (representing live cells) cells were sorted for in vivo tumorigenesis study in Fig. 3H.|
|sc-12-0203_sm_SupplFigure3.pdf||168K||Figure S3. Supervised hierarchical clustering of 1,196 differentially expressed transcripts between ALDH+ and ALDH- cells from xenografted patient melanomas (MF347, MB929 and MB947m). Colored spots indicate upregulated (red) or downregulated (blue) genes.|
|sc-12-0203_sm_SupplFigure4.pdf||168K||Figure S4. qRT-PCR analysis of ADAR, CDC42 and USH1C in 1205Lu cells. (A) Expression of mRNAs in ALDH7plus; and ALDH− subpopulation of 1205Lu cells. (B) Expression of mRNAs after knockdown of ALDH1A3 (si-1A3) in ALDH+1205Lu cells. Expression levels were normalized to GAPDH. Data represent mean ± SEM (n=3). *, P < 0.05; **, P < 0.01; ***, P < 0.001 compared with transfection of control siRNA (si-ctrl).|
|sc-12-0203_sm_SupplTable1.pdf||168K||Supplemental Table 1.|
|sc-12-0203_sm_SupplTable2.pdf||168K||Supplemental Table 2.|
|sc-12-0203_sm_SupplTable3.pdf||168K||Supplemental Table 3.|
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