Additional Supporting Information may be found in the online version of this article.

sc-12-0203_sm_SupplFigure1.pdf168KFigure S1. Expression of ALDH isoforms in human melanoma cell lines. The copy number of human ALDH1A1 (1A1) and ALDH1A3 (1A3) was analyzed by qRT-PCR. mRNAs of ALDH+ and ALDH- subpopulations obtained from human melanoma cell lines (1205Lu, A375, WM239A and HS294T) were analyzed. Copy number was counted and determined by standard curve analysis.
sc-12-0203_sm_SupplFigure2.pdf168KFigure S2. Representative flow cytometric analysis of GFP-expressing cells. ALDH+1205Lu cells were transfected with GFP-shRNA control plasmid (sh-ctrl) or GFP-shRNA-ALDH1A3 plasmid (sh-1A3). GFP-positive (representing successful transfection) and DAPI-negative (representing live cells) cells were sorted for in vivo tumorigenesis study in Fig. 3H.
sc-12-0203_sm_SupplFigure3.pdf168KFigure S3. Supervised hierarchical clustering of 1,196 differentially expressed transcripts between ALDH+ and ALDH- cells from xenografted patient melanomas (MF347, MB929 and MB947m). Colored spots indicate upregulated (red) or downregulated (blue) genes.
sc-12-0203_sm_SupplFigure4.pdf168KFigure S4. qRT-PCR analysis of ADAR, CDC42 and USH1C in 1205Lu cells. (A) Expression of mRNAs in ALDH7plus; and ALDH− subpopulation of 1205Lu cells. (B) Expression of mRNAs after knockdown of ALDH1A3 (si-1A3) in ALDH+1205Lu cells. Expression levels were normalized to GAPDH. Data represent mean ± SEM (n=3). *, P < 0.05; **, P < 0.01; ***, P < 0.001 compared with transfection of control siRNA (si-ctrl).
sc-12-0203_sm_SupplMaterials.pdf313KSupplemental Data
sc-12-0203_sm_SupplTable1.pdf168KSupplemental Table 1.
sc-12-0203_sm_SupplTable2.pdf168KSupplemental Table 2.
sc-12-0203_sm_SupplTable3.pdf168KSupplemental Table 3.

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