SEARCH

SEARCH BY CITATION

Additional Supporting Information may be found in the online version of this article.

FilenameFormatSizeDescription
sc-11-1157_sm_SupplFigure1.jpg797KSupplemental Figure 1: Cell proliferation in the anterior and posterior SVZ following neonatal HI injury. (A-C) Cell proliferation in the SVZ following HI. Coronal sections of the aSVZ and pSVZ at P10 (5 days after HI) were immunostained for Ki67 (A, White broken lines indicate the wall of the lateral ventricle). The percentages of Ki67+ cells that were Ki67+Olig2+ newly generated OPCs were 33.8±4.3% (aSVZ) and 39.6±3.4% (pSVZ). The density of Ki67+ proliferating cells in the SVZ of the HI–injured hemisphere was significantly greater than that in the intact mice at P10 and P12 (B), (n=3, *P <0.05, **P <0.01, unpaired t-test) and showed significant increases in both the aSVZ and pSVZ at P10 (C), (n=3, *P <0.05, unpaired t-test). (D) Density of newly generated OPCs in the aSVZ. There was no significant difference in the density of BrdU+Olig2+ cells between the intact and HI-injured aSVZ at any time-point (P10, P13, or P17). Scale bar: 100 μm.
sc-11-1157_sm_SupplFigure2.jpg312KSupplemental Figure 2: Decreased number of proliferating Olig2+ cells in the SVZ during normal neonatal development. The density of BrdU+ newly generated Olig2+ cells in the aSVZ (A) and pSVZ (B). BrdU was intraperitoneally injected into wild-type mice 2 h before sacrifice, at P3, P5, P7, P10, P13, and P17. The densities of BrdU+Olig2+ cells at P3 were significantly higher than those at later stages in both the aSVZ and pSVZ. (n=3, *P <0.05, one-way ANOVA with Bonferroni's multiple comparison test).
sc-11-1157_sm_SupplFigure3.jpg338KSupplemental Figure 3: Differentiation of CFP+ cells into neuronal progenitors and astrocytes in the CC of Olig2CreERTM; Rosa26ECFP mice after HI. Brain sections of P13, P17, and P24 Olig2CreERTM; Rosa26ECFP mice exposed to HI at P5 and tamoxifen injection at P9 were immunostained for the new neuron marker Dcx or astrocyte marker GFAP. The percentage of CFP+ cells co-labeled with Dcx (A) or GFAP (B) in the CC was less than 10% at any time point, with no significant difference between the HI-exposed and intact groups.
sc-11-1157_sm_SupplFigure4.jpg85KSupplemental Figure 4: Knockdown of erythropoietin receptor (EPOR) by siRNA transfection in OPC culture. Q-RT-PCR for EPOR mRNA in OPCs cultured with negative control siRNA or EPOR-specific siRNA. Expression levels were normalized to those of GAPDH. The EPOR mRNA expression was suppressed significantly in the EPOR-specific siRNA-added OPC culture compared to that with negative control siRNA (n=5, *P<0.05, one-way ANOVA with Bonferroni's multiple comparison test).
sc-11-1157_sm_SupplMaterial.pdf119KSupplemental Data

Please note: Wiley Blackwell is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.