SEARCH

SEARCH BY CITATION

Additional Supporting Information may be found in the online version of this article.

FilenameFormatSizeDescription
sc-12-0485_sm_SupplTable1.pdf79KSupplementary Table 1
sc-12-0485_sm_SupplTable2.pdf23KSupplementary Table 2
sc-12-0485_sm_SupplFigure1.tif2932KSupplemental Figure S1. Transplanted UCB ALDHhi cells induced transient recovery of perfusion in the ischemic limbs of mice that received preparative irradiation. Representative LDPI following right femoral artery ligation, 300 cGy sublethal preparative irradiation to suppress immune rejection, and tail vein injection with (A) PBS vehicle control (n=6), (B) 20x106 unsorted UCB MNC (n=7), (C) 2x105 UCB ALDHhi cells (n=8), or (D) 10x106 ALDHlo cells (n=8), and monitored for 28 days. Numbers in lower left of each LDPI image indicate perfusion ratio of the ischemic versus the non-ischemic hindlimb from ankle to toe. (E) Summary of mean perfusion ratios in all transplanted mice as described above. Compared to all other treatments, transplantation of UCB ALDHhi cells promoted a transient recovery of perfusion in ischemic mouse hindlimbs at day 21 post-transplantation. Data are expressed as mean ± SEM, **p<0.01.
sc-12-0485_sm_SupplFigure2.tif2932KSupplemental Figure S2. Transplanted UCB ALDHhi cells augmented blood vessel density in the ischemic hindlimb of irradiated mice. Representative photomicrographs of adductor muscle sections from the non-ischemic and ischemic hindlimbs of mice injected with (A, B) PBS or (C) UCB ALDHhi cells or (D) UCB ALDHlo cells stained for vWF+ blood vessels at day 28 post-transplantation. Scale bar = 100μm. (E) Summary of vWF+ blood vessel density for each transplanted cohort at 28 days posttransplantation. Compared to all other treatments, blood vessel density was increased in the ischemic limb of mice transplanted with human UCB ALDHhi cells. Data are expressed as mean ± SEM, ***p<0.001.
sc-12-0485_sm_SupplFigure3.tif2932KSupplemental Figure S3. Transplanted UCB ALDHhi cell showed reduced engraftment in the ischemic limb and increased engraftment in the bone marrow UCB ALDHhi cells promote limb revascularization Putman et al. of irradiated mice. (A,B) Representative flow cytometric detection of human cells in (A) bone marrow or (B) ischemic muscle (B) of mice transplanted with UCB ALDHhi cells after sublethal (300cGy) preparative irradiation. (C-D) Summary of human cell (CD45+/HLA-A,B,C+) engraftment in (C) the bone marrow or (D) ischemic muscle of all transplanted mice at 7 and 28 days–post transplantation. (E, F) Representative photomicrographs showing the detection of transplanted human cells (red) in the adductor muscle of the ischemic hindlimb of mice transplanted with UCB ALDHhi cells at 7 and 28 days post-transplantation. GUSB+ human cells (arrows) were detected in the ischemic limb of mice at 28 days post-transplantation, Scale bar = 100μm.

Please note: Wiley Blackwell is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.