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Tissue-Specific Stem Cells
Article first published online: 22 OCT 2012
Copyright © 2012 AlphaMed Press
Volume 30, Issue 11, pages 2487–2497, November 2012
How to Cite
Paik, D. Y., Janzen, D. M., Schafenacker, A. M., Velasco, V. S., Shung, M. S., Cheng, D., Huang, J., Witte, O. N. and Memarzadeh, S. (2012), Stem-Like Epithelial Cells Are Concentrated in the Distal End of the Fallopian Tube: A Site for Injury and Serous Cancer Initiation. STEM CELLS, 30: 2487–2497. doi: 10.1002/stem.1207
Author contributions: D.Y.P. and D.M.J.: conception and design, collection and/or assembly of data, data analysis and interpretation, and manuscript writing; A.M.S., V.S.V., M.S.S., and D.C.: conception and design, collection and/or assembly of data, and data analysis and interpretation; J.H.: data analysis and interpretation; O.N.W.: manuscript writing; S.M.: conception and design, data analysis and interpretation, manuscript writing, and final approval of manuscript.
Disclosure of potential conflicts of interest is found at the end of this article.
First published online in STEM CELLSEXPRESS August 21, 2012.
- Issue published online: 22 OCT 2012
- Article first published online: 22 OCT 2012
- Accepted manuscript online: 21 AUG 2012 08:21AM EST
- Manuscript Accepted: 11 JUL 2012
- Manuscript Received: 5 MAY 2012
- Liz Tilberis Scholars Program
- Ovarian Cancer Research Fund, Inc.
- Gynecologic Cancer Foundation
- St. Louis Ovarian Cancer Awareness Research Grant
- Ovarian Cancer Circle
- Howard Hughes Medical Institute investigator
- Adult stem cells;
- Clonal assays;
- Tissue-specific stem cells
The reproductive role of the fallopian tube is to transport the sperm and egg. The tube is positioned to act as a bridge between the ovary where the egg is released and the uterus where implantation occurs. Throughout reproductive years, the fallopian tube epithelium undergoes repetitive damage and regeneration. Although a reservoir of adult epithelial stem cells must exist to replenish damaged cells, they remain unidentified. Here, we report isolation of a subset of basally located human fallopian tube epithelia (FTE) that lack markers of ciliated (β-tubulin; TUBB4) or secretory (PAX8) differentiated cells. These undifferentiated cells expressed cell surface antigens: epithelial cell adhesion molecule, CD44, and integrin α 6. This FTE subpopulation was fivefold enriched for cells capable of clonal growth and self-renewal suggesting that they contain the FTE stem-like cells (FTESCs). A twofold enrichment of the FTESC was found in the distal compared to the proximal end of the tube. The distal fimbriated end of the fallopian tube is a well-characterized locus for initiation of serous carcinomas. An expansion of the cells expressing markers of FTESC was detected in tubal intraepithelial carcinomas and in fallopian tubes from patients with invasive serous cancer. These findings suggest that FTESC may play a role in the initiation of serous tumors. Characterization of these stem-like cells will provide new insight into how the FTE regenerate, respond to injury, and may initiate cancer. STEM CELLS2012;30:2487–2497