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Additional Supporting Information may be found in the online version of this article.

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sc-12-0534_sm_SupplFigure1.tif374KSupplementary Figure 1: Differential expression of Class 1-IV HDACs during pluripotency and differentiation. A. mRNA expression levels of class I HDACs in pluripotent mES cells and differentiating cells at various days of differentiation. B. mRNA expression levels of class IIA HDACs in pluripotent mES cells and differentiating cells at various days of differentiation. C. mRNA expression levels of class IIB HDACs in pluripotent mES cells and differentiating cells at various days of differentiation. D. mRNA expression levels of class III HDACs in pluripotent mES cells and differentiating cells at various days of differentiation. E. mRNA expression levels of class IV HDACs in pluripotent mES cells and differentiating cells at different days of differentiation.
sc-12-0534_sm_SupplFigure2.tif1193KSupplementary Figure 2: Loss of HDAC1 does not affect the expression levels of other HDACs but reduces the expression of endodermal and ectodermal lineage genes. A. Levels of HDAC2 and HDAC5 mRNA in iPS and mES cells and their respective HDAC1-KD cells. B. Number of divisions as time progresses for mES, iPS and respective HDAC1 knock down cells. C. Division time mES, iPS and respective HDAC1 knock down cells. D. Light microscopy images showing lack of differentiation in EBs derived from mES and iPS cells lacking HDAC1 at day 6 of differentiation. Black arrows indicates distance from the center of the EB to the periphery. E. Quantitative RTPCR showing mRNA expression levels of early endodermal and ectodermal genes in wt mES and iPS cells and respective HDAC1-KD cells. F. Western Blot for OCT4 following immunoprecipitation with HDAC1 showing physical association of OCT4 with HDAC1 in mES and iPS cells.
sc-12-0534_sm_SupplTable1.tif24KSupplementary Table 1. Sequences of primers used for pyrosequencing. Hoxha et al, SC-12-0534-R1
sc-12-0534_sm_SupplVideo1.mov2867KS.V1-S.V2. Embryoid Bodies derived from iPS-HDAC1-KD cells show delayed/reduced beating. EBs derived from: SV1. iPS cells; SV2. iPS-HDAC1-KD cells.
sc-12-0534_sm_SupplVideo2.mov2804KS.V1-S.V2. Embryoid Bodies derived from iPS-HDAC1-KD cells show delayed/reduced beating. EBs derived from: SV1. iPS cells; SV2. iPS-HDAC1-KD cells.
sc-12-0534_sm_SupplVideo3.avi15647KS.V3-S.V4. Cells derived from iPS cells lacking HDAC1 are not synchronized and do not respond to external stimuli. EBs derived from: SV5. iPS cells and SV6. iPS-HDAC1- KD cells. Calcium uptake and release (flashes of red) were recorded during spontaneous contraction followed by stimulation at 350ms and then ceasing of external stimulus.
sc-12-0534_sm_SupplVideo4.avi21870KS.V3-S.V4. Cells derived from iPS cells lacking HDAC1 are not synchronized and do not respond to external stimuli. EBs derived from: SV5. iPS cells and SV6. iPS-HDAC1- KD cells. Calcium uptake and release (flashes of red) were recorded during spontaneous contraction followed by stimulation at 350ms and then ceasing of external stimulus.

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