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Tissue-Specific Stem Cells
Version of Record online: 22 OCT 2012
Copyright © 2012 AlphaMed Press
Volume 30, Issue 11, pages 2535–2547, November 2012
How to Cite
Hou, Y., Ouyang, X., Wan, R., Cheng, H., Mattson, M. P. and Cheng, A. (2012), Mitochondrial Superoxide Production Negatively Regulates Neural Progenitor Proliferation and Cerebral Cortical Development. STEM CELLS, 30: 2535–2547. doi: 10.1002/stem.1213
Author contributions: Y.H., M.P.M., and A.C.: designed the study; Y.H., X.O., R.W., and A.C.: performed the experiments; Y.H. and A.C.: analyzed the data; H.C.: contributed reagents; Y.H, H.C., M.P.M., and A.C.: wrote the manuscript.
Disclosure of potential conflicts of interest is found at the end of this article.
First published online in STEM CELLSEXPRESS September 4, 2012.
- Issue online: 22 OCT 2012
- Version of Record online: 22 OCT 2012
- Accepted manuscript online: 4 SEP 2012 10:02AM EST
- Manuscript Accepted: 25 JUL 2012
- Manuscript Received: 9 APR 2012
- Intramural Research Program of the National Institute on Aging
- National Natural Science Foundation
- National Basic Research Program of China. Grant Number: 2011CB809100
Additional Supporting Information may be found in the online version of this article.
|sc-12-0342_sm_SupplFigure1.TIF||1097K||Figure S1. Mitochondrial morphology in cultured cortical neurons (8 days in culture). The primary cortical neurons were co-transfected with plasmid eGFP and mt-Dred. Mitochondria are distributed throughout dendrites of the cell, with particularly large numbers of mitochondria in the cell body Bar = 20 μm.|
|sc-12-0342_sm_SupplFigure2.tif||919K||Figure S2. Reduction of SO flash incidence under 5% O2 promotes NPCs proliferation. (A): SO flash incidence (percentage of cells with superoxide flash activity within 3 minutes of time-lapse imaging) plotted as percentage of control condition mean value (n = 3 separate experiments). *p<0.05 and **p<0.001compared to the control value. (B, C): Results of quantification of neurosphere number (B) and diameter size (C) in cultures maintained for 6 d under 21% O2 or 5% O2. (D): quantitative analysis of proliferation index (BrdU+ cells/total cells) in dissociated NPC cultures treated for 24 h under 21% O2 or 5% O2. Values are expressed as a percentage of the mean of the control condition (n = 3 separate experiments) *p<0.05, **p<0.001.|
|sc-12-0342_sm_SupplMovie1.avi||27301K||Supplementary Movie 1|
|sc-12-0342_sm_SupplMovie2.avi||67082K||Supplementary Movie 2|
|sc-12-0342_sm_SupplMovie3.avi||61856K||Supplementary Movie 3|
|sc-12-0342_sm_SupplMovie4.avi||78003K||Supplementary Movie 4|
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