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Additional Supporting Information may be found in the online version of this article.

FilenameFormatSizeDescription
sc-12-0363_sm_SupplFigure1.pdf295KSupplementary Figure 1: Blood vessel morphology and distribution in the non-irradiated left and irradiated right SVZ one month post-irradiation. A) Double labeling of DCx (green) and CD31 (red) in SVZ shows no difference in blood vessel distribution. B) Electron micrographs of blood vessels (BV) in the left and irradiated right SVZ showing endothelial cells (*) and basal lamina (arrows). C) Bar graph showing no difference in the number of blood vessels in the left and irradiated right SVZ. D) Bar graph showing no difference in the size of blood vessels in the left and irradiated right SVZ. Scale bars: 100μm (A), 200nm (B).
sc-12-0363_sm_SupplFigure2.pdf532KSupplementary Figure 2: Disruption of the migration of neuroblasts one week after localized irradiation. A) Anterior dorsal SVZ is targeted with a lateral beam of radiation, indicated by a red arrow. B) Whole mount of SVZ (AD-anterior dorsal). C) SVZ immunostained for GFAP (green) and DCx (red). (C′&C″) Migrating neuroblasts were eliminated, and cells from the posterior SVZ did not migrate through the irradiated region, shown at higher magnification. Scale bars: 100μm (C′-C″).
sc-12-0363_sm_SupplFigure3.pdf117KSupplementary Figure 3: Quantification of proliferating Ki67+ cells in the OB, RMS, SVZ regions of the brain in sham (green bars), OB/RMS (blue bars) and OB/RMS/aSVZ (red bars) irradiated mice a week after GFP expressing retroviral injections. There is a significant reduction in the Ki67+ cells in the RMS and OB regions of the brain in OB/RMS and OB/RMS/aSVZ irradiated mice. There is no difference in the number of Ki67+ cells in the SVZ of OB/RMS and sham irradiated mice, indicating that the SVZ in the OB/RMS irradiated is not affected with radiation. The Ki67+ cells in the SVZ of OB/RMS/aSVZ region are from the posterior SVZ that did not receive irradiation. *p<0.05 vs sham.
sc-12-0363_sm_SupplTable1.tiff154KSupplementary Table 1
sc-12-0363_sm_SupplTable2.tiff161KSupplementary Table 2

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