Telephone: 410-502-2869; Fax: 410-502-7559
Tissue-Specific Stem Cells
Version of Record online: 22 OCT 2012
Copyright © 2012 AlphaMed Press
Volume 30, Issue 11, pages 2548–2560, November 2012
How to Cite
Achanta, P., Capilla-Gonzalez, V., Purger, D., Reyes, J., Sailor, K., Song, H., Garcia-Verdugo, J. M., Gonzalez-Perez, O., Ford, E. and Quinones-Hinojosa, A. (2012), Subventricular Zone Localized Irradiation Affects the Generation of Proliferating Neural Precursor Cells and the Migration of Neuroblasts. STEM CELLS, 30: 2548–2560. doi: 10.1002/stem.1214
Author contributions: P.A.: conception and design of experiments, data collection, assembly, analysis and interpretation, manuscript writing, final approval of manuscript, and contributed to the manuscript editing; D.P.: data collection, assembly, analysis, and contributed to the manuscript editing; J.R.: data collection and contributed to the manuscript editing; V.C.G. and J.M.G.V.: data collection, assembly, analysis and interpretation from electron microscopy, and contributed to the manuscript editing; K.S. and H.S.: provided the GFP expressing retrovirus, assisted with stereotactic injections, and contributed to the manuscript editing; O.G.P.: experimental design, data interpretation, and contributed to the manuscript editing; E.F.: conception and design of experiments, SARPP technology, data interpretation, and contributed to the manuscript editing; A.Q.H.: conception and design of experiments, final approval of manuscript, financial support, and contributed to the manuscript editing.
Disclosure of potential conflicts of interest is found at the end of this article.
First published online in STEM CELLSEXPRESS September 4, 2012.
- Issue online: 22 OCT 2012
- Version of Record online: 22 OCT 2012
- Accepted manuscript online: 4 SEP 2012 10:01AM EST
- Manuscript Accepted: 21 JUL 2012
- Manuscript Received: 13 APR 2012
- Maryland Stem Cell Research Fund
- Robert Wood Johnson Foundation
- Howard Hughes Medical Institute
- National Institutes of Health
- Consejo Nacional de Ciencia y Tecnologia's. Grant Number: CONACyT, CB-2008-101476
- Prometeo. Grant Number: GVPROMETEO-2009/011
- Department of Radiation Oncology, University of Washington Medical Center, Seattle, Washington, DC
Additional Supporting Information may be found in the online version of this article.
|sc-12-0363_sm_SupplFigure1.pdf||295K||Supplementary Figure 1: Blood vessel morphology and distribution in the non-irradiated left and irradiated right SVZ one month post-irradiation. A) Double labeling of DCx (green) and CD31 (red) in SVZ shows no difference in blood vessel distribution. B) Electron micrographs of blood vessels (BV) in the left and irradiated right SVZ showing endothelial cells (*) and basal lamina (arrows). C) Bar graph showing no difference in the number of blood vessels in the left and irradiated right SVZ. D) Bar graph showing no difference in the size of blood vessels in the left and irradiated right SVZ. Scale bars: 100μm (A), 200nm (B).|
|sc-12-0363_sm_SupplFigure2.pdf||532K||Supplementary Figure 2: Disruption of the migration of neuroblasts one week after localized irradiation. A) Anterior dorsal SVZ is targeted with a lateral beam of radiation, indicated by a red arrow. B) Whole mount of SVZ (AD-anterior dorsal). C) SVZ immunostained for GFAP (green) and DCx (red). (C′&C″) Migrating neuroblasts were eliminated, and cells from the posterior SVZ did not migrate through the irradiated region, shown at higher magnification. Scale bars: 100μm (C′-C″).|
|sc-12-0363_sm_SupplFigure3.pdf||117K||Supplementary Figure 3: Quantification of proliferating Ki67+ cells in the OB, RMS, SVZ regions of the brain in sham (green bars), OB/RMS (blue bars) and OB/RMS/aSVZ (red bars) irradiated mice a week after GFP expressing retroviral injections. There is a significant reduction in the Ki67+ cells in the RMS and OB regions of the brain in OB/RMS and OB/RMS/aSVZ irradiated mice. There is no difference in the number of Ki67+ cells in the SVZ of OB/RMS and sham irradiated mice, indicating that the SVZ in the OB/RMS irradiated is not affected with radiation. The Ki67+ cells in the SVZ of OB/RMS/aSVZ region are from the posterior SVZ that did not receive irradiation. *p<0.05 vs sham.|
|sc-12-0363_sm_SupplTable1.tiff||154K||Supplementary Table 1|
|sc-12-0363_sm_SupplTable2.tiff||161K||Supplementary Table 2|
Please note: Wiley Blackwell is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.