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Additional Supporting Information may be found in the online version of this article.

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sc-12-0427_sm_SupplFigure1.pdf284KSupplemental Figure 1. MSC display changes in markers for differentiation as well as cell surface receptors during progress of differentiation. Change in levels of osteogenic markers during progress of prhMSC osteogenic differentiation on tissue culture plastic (A). Change in level of adipogenic markers by qPCR during adipogenic differentiation of prhMSC on tissue culture plastic (B). Change in nuclear size during progress of adipogenic differentiation of imhMSC on tissue culture plastic (C). Change in the expression of EGFR and Fas in prhMSC during adipogenic (D) and osteogenic differentiation (E) on tissue culture plastic.
sc-12-0427_sm_SupplFigure2.pdf588KSupplemental Figure 2. Time course of death of MSC in the presence of FasL. Fluorochrome inhibitor of caspase assay (FLICA) stained imhMSC (A) and prhMSC (B) after treatment with FasL for defined time-points. Shown are representative photomicrographs of cells of two independent experiments.
sc-12-0427_sm_SupplFigure3.pdf173KSupplemental Figure 3. Time course of death of differentiating adipocytes in the presence of FasL. Fluorochrome inhibitor of caspase assay (FLICA) and Hoescht 33342 images of prhMSC in adipogenic media on Day 15 (A) and Day 30, overlaid with phase contrast image to identify fat droplets (B) treated with FasL for 0, 8 and 24 hours. Shown are representative photomicrographs of cells of two independent experiments.

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