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Version of Record online: 19 DEC 2012
Copyright © 2012 AlphaMed Press
Volume 31, Issue 1, pages 104–116, January 2013
How to Cite
Rodrigues, M., Blair, H., Stockdale, L., Griffith, L. and Wells, A. (2013), Surface Tethered Epidermal Growth Factor Protects Proliferating and Differentiating Multipotential Stromal Cells from FasL-Induced Apoptosis. STEM CELLS, 31: 104–116. doi: 10.1002/stem.1215
Author contributions: M.R.: conception and design, performed research, data analysis and interpretation, and wrote manuscript; H.B.: data analysis and image processing; L.S.: prepared tEGF surfaces; L.G.: conception and design, data interpretation, and financial support; A.W.: conception and design, data interpretation, edited manuscript, and financial support.
Disclosure of potential conflicts of interest is found at the end of this article.
First published online in STEM CELLSEXPRESS September 4, 2012.
- Issue online: 19 DEC 2012
- Version of Record online: 19 DEC 2012
- Accepted manuscript online: 4 SEP 2012 10:02AM EST
- Manuscript Accepted: 25 JUL 2012
- Manuscript Received: 8 MAY 2012
- National Institute of General Medical Sciences
- National Institute of Dental and Craniofacial Research. Grant Numbers: GM069668, DE019523
Additional Supporting Information may be found in the online version of this article.
|sc-12-0427_sm_SupplFigure1.pdf||284K||Supplemental Figure 1. MSC display changes in markers for differentiation as well as cell surface receptors during progress of differentiation. Change in levels of osteogenic markers during progress of prhMSC osteogenic differentiation on tissue culture plastic (A). Change in level of adipogenic markers by qPCR during adipogenic differentiation of prhMSC on tissue culture plastic (B). Change in nuclear size during progress of adipogenic differentiation of imhMSC on tissue culture plastic (C). Change in the expression of EGFR and Fas in prhMSC during adipogenic (D) and osteogenic differentiation (E) on tissue culture plastic.|
|sc-12-0427_sm_SupplFigure2.pdf||588K||Supplemental Figure 2. Time course of death of MSC in the presence of FasL. Fluorochrome inhibitor of caspase assay (FLICA) stained imhMSC (A) and prhMSC (B) after treatment with FasL for defined time-points. Shown are representative photomicrographs of cells of two independent experiments.|
|sc-12-0427_sm_SupplFigure3.pdf||173K||Supplemental Figure 3. Time course of death of differentiating adipocytes in the presence of FasL. Fluorochrome inhibitor of caspase assay (FLICA) and Hoescht 33342 images of prhMSC in adipogenic media on Day 15 (A) and Day 30, overlaid with phase contrast image to identify fat droplets (B) treated with FasL for 0, 8 and 24 hours. Shown are representative photomicrographs of cells of two independent experiments.|
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