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Tissue-Specific Stem Cells
Article first published online: 27 NOV 2012
Copyright © 2012 AlphaMed Press
Volume 30, Issue 12, pages 2774–2783, December 2012
How to Cite
Strong, A. L., Semon, J. A., Strong, T. A., Santoke, T. T., Zhang, S., McFerrin, H. E., Gimble, J. M. and Bunnell, B. A. (2012), Obesity-Associated Dysregulation of Calpastatin and MMP-15 in Adipose-Derived Stromal Cells Results in their Enhanced Invasion. STEM CELLS, 30: 2774–2783. doi: 10.1002/stem.1229
Author contributions: A.L.S.: conception and design, collection and assembly of data, data analysis and interpretation, and manuscript writing; J.A.S.: conception and design and data analysis and interpretation; T.A.S., T.T.S., and S.Z.: collection and assembly of data; H.E.M. and J.M.G.: conception and design, financial support, and data analysis and interpretation; B.A.B.: conception and design, financial support, data analysis and interpretation, and final approval of manuscript.
Disclosure of potential conflicts of interest is found at the end of this article.
First published online in STEM CELLSEXPRESS November 7, 2012.
- Issue published online: 27 NOV 2012
- Article first published online: 27 NOV 2012
- Accepted manuscript online: 11 SEP 2012 01:56PM EST
- Manuscript Accepted: 21 AUG 2012
- Manuscript Received: 28 JUN 2012
- Tulane University (B.A.B.) and the Pennington Biomedical Research Center (J.M.G.)
- National Center for Research Resources. Grant Number: (5P20RR016456-11)
- National Institute of General Medical Sciences. Grant Number: (8 P20GM103424-11)
- National Institutes of Health
Additional Supporting Information may be found in the online version of this article.
|sc-12-0599_sm_SupplMaterial1.pdf||25K||Supplemental Material 1. Donor demographics.|
|sc-12-0599_sm_SupplMaterial2.pdf||42K||Supplemental Material 2. Primer sets used in amplification of ASC mRNA regions.|
|sc-12-0599_sm_SupplMaterial3.pdf||265K||Supplemental Material 3. RT-PCR analyses of MMP, TIMP, calpain, and calpastatin from different ASC populations. RNA was extracted from ASCs primed with breast cancer conditioned media. Analysis of RNA expression of ASCs was analyzed by gel electrophoresis. β-actin is shown as a standard reference. Primer sets are listed in Supplemental Material 2.|
|sc-12-0599_sm_SupplMaterial4.pdf||448K||Supplemental Material 4. Quantification of RT-PCR analyses of MMP, TIMP, calpain, and calpastatin. Densitometry was performed on RT-PCR gel electrophoresis and represented as a ratio relative to β-actin. Bars, ± SD. *P < .05.|
|sc-12-0599_sm_SupplMaterial5.pdf||263K||Supplemental Material 5. Western blot analyses ASCs isolated from 24 donors for MMP-15, calpain-4, and calpastatin expression. Protein lysate was isolated from ASCs isolated from 24 donors and primed with breast cancer conditioned media. Where indicated, samples isolated from donors with the same obesity status or depot site were pooled together for analysis. A total of 20 μg of protein was separated by SDS-PAGE under reducing conditions, blotted, and probed for MMP-15, calpain-4, and calpastatin. Bars, ± SD. *P < .05|
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