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Embryonic Stem Cells/Induced Pluripotent Stem Cells
Article first published online: 27 NOV 2012
Copyright © 2012 AlphaMed Press
Volume 30, Issue 12, pages 2683–2691, December 2012
How to Cite
Muñoz Descalzo, S., RuÉ, P., Garcia-Ojalvo, J. and Arias, A. M. (2012), Correlations Between the Levels of Oct4 and Nanog as a Signature for Naïve Pluripotency in Mouse Embryonic Stem Cells. STEM CELLS, 30: 2683–2691. doi: 10.1002/stem.1230
Author contributions: S.M.D.: conceived the project, carried out the experiments, the measurements, and the analysis of the data, and wrote the manuscript; A.M.A.: conceived the project and wrote the manuscript; P.R. and J.G.O.: contributed to the analysis of the data.
Disclosure of potential conflicts of interest is found at the end of this article.
First published online in STEM CELLSEXPRESS November 7, 2012.
- Issue published online: 27 NOV 2012
- Article first published online: 27 NOV 2012
- Accepted manuscript online: 11 SEP 2012 02:07PM EST
- Manuscript Accepted: 19 AUG 2012
- Manuscript Received: 29 MAY 2012
- ICREA foundation. P.R. is supported by a FI grant from the Generalitat de Catalunya
- Embryonic stem cells;
- Pluripotent stem cells;
- Transcription factors
The pluripotent state is traditionally associated with large absolute levels of certain transcription factors such as Nanog and Oct4. Here, we present experimental observations using quantitative immunofluorescence that pluripotency in mouse embryonic stem cells (mESCs) is established by specific ratios between Oct4 and Nanog. When cells are grown in 2i conditions, they exhibit uniform levels of pluripotency and this is associated with a high correlation between the levels of Oct4 and Nanog in individual cells. The correlation is lost when cells differentiate. Our results suggest that the correlation between these two factors and the distribution of Oct4/Nanog ratios can be used as quantifiers to distinguish between three subpopulations in an mESC culture: pluripotent, lineage-primed, and differentiating cells. When we apply these quantifiers to cells with lower levels of Nanog or mutant for β-Catenin or Tcf3, the results suggest that these cells exhibit higher probability of differentiation. STEM CELLS 2012;30:2683–2691