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Additional Supporting Information may be found in the online version of this article.

FilenameFormatSizeDescription
sc-12-0454_sm_SupplFigure1.tif416KSuppl. Fig 1. Spheroid cultures were sorted for 10% highest and 10% lowest expression of LGR5 and stained for Ki67. The scale bar indicates 20 μm.
sc-12-0454_sm_SupplFigure2.tif200KSuppl. Fig 2. Spheroid cultures were sorted for 10% highest and 10% lowest expression of LGR5 (left). Purity of Lgrlow cells was checked by immediately re-running fraction of Lgr5low sorted cells (right)
sc-12-0454_sm_SupplFigure3.tif109KSuppl. Fig 3. Lentiviral construct targeting LGR5 (shB4 and shB5) led to efficient knockdown in spheroid cultures.

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