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Cancer Stem Cells
Article first published online: 22 OCT 2012
Copyright © 2012 AlphaMed Press
Volume 30, Issue 11, pages 2378–2386, November 2012
How to Cite
Kemper, K., Prasetyanti, P. R., De Lau, W., Rodermond, H., Clevers, H. and Medema, J. P. (2012), Monoclonal Antibodies Against Lgr5 Identify Human Colorectal Cancer Stem Cells. STEM CELLS, 30: 2378–2386. doi: 10.1002/stem.1233
Author contributions: K.K.: conception and design, collection and/or assembly of data, data analysis and interpretation, and manuscript writing; W.D.L.: conception and design and provision of study material; P.R.P.: provision of study material, collection and/or assembly of data, and data analysis and interpretation.; H.R.: collection and/or assembly of data; H.C.: conception and design and final approval of manuscript; J.P.M.: conception and design, data analysis and interpretation, and final approval of manuscript.
Disclosure of potential conflicts of interest is found at the end of this article.
First published online in STEM CELLSEXPRESS August 7, 2012.
- Issue published online: 22 OCT 2012
- Article first published online: 22 OCT 2012
- Accepted manuscript online: 11 SEP 2012 02:04PM EST
- Manuscript Accepted: 13 AUG 2012
- Manuscript Received: 12 MAY 2012
- AMC graduate school scholarship
- VICI grant of NWO
- Dutch Cancer Society. Grant Number: UvA 2009-4416
Additional Supporting Information may be found in the online version of this article.
|sc-12-0454_sm_SupplFigure1.tif||416K||Suppl. Fig 1. Spheroid cultures were sorted for 10% highest and 10% lowest expression of LGR5 and stained for Ki67. The scale bar indicates 20 μm.|
|sc-12-0454_sm_SupplFigure2.tif||200K||Suppl. Fig 2. Spheroid cultures were sorted for 10% highest and 10% lowest expression of LGR5 (left). Purity of Lgrlow cells was checked by immediately re-running fraction of Lgr5low sorted cells (right)|
|sc-12-0454_sm_SupplFigure3.tif||109K||Suppl. Fig 3. Lentiviral construct targeting LGR5 (shB4 and shB5) led to efficient knockdown in spheroid cultures.|
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