Brief Report: Adult Hippocampal Precursor Cells Shorten S-Phase and Total Cell Cycle Length During Neuronal Differentiation§

Authors

  • Moritz D. Brandt,

    Corresponding author
    1. Division of Neurodegenerative Diseases, Department of Neurology, Dresden University of Technology, Dresden, Germany
    2. German Center for Neurodegenerative Diseases (DZNE) Dresden, Dresden, Germany
    3. Center for Regenerative Therapies Dresden (CRTD), Dresden University of Technology, Dresden, Germany
    • Moritz D. Brandt, Division of Neurodegenerative Diseases, Department of Neurology, Dresden University of Technology, Fetscherstraβe 74, 01307 Dresden, Germany

      Alexander Storch, Division of Neurodegenerative Diseases, Department of Neurology, Dresden University of Technology, Fetscherstraβe 74, 01307 Dresden, Germany

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    • Telephone: ++49-351-458-2532; Fax: ++49-351-458-4352

  • Matthias Hübner,

    1. Division of Neurodegenerative Diseases, Department of Neurology, Dresden University of Technology, Dresden, Germany
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  • Alexander Storch

    Corresponding author
    1. Division of Neurodegenerative Diseases, Department of Neurology, Dresden University of Technology, Dresden, Germany
    2. German Center for Neurodegenerative Diseases (DZNE) Dresden, Dresden, Germany
    3. Center for Regenerative Therapies Dresden (CRTD), Dresden University of Technology, Dresden, Germany
    • Moritz D. Brandt, Division of Neurodegenerative Diseases, Department of Neurology, Dresden University of Technology, Fetscherstraβe 74, 01307 Dresden, Germany

      Alexander Storch, Division of Neurodegenerative Diseases, Department of Neurology, Dresden University of Technology, Fetscherstraβe 74, 01307 Dresden, Germany

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    • Telephone: ++49-351-458-2532; Fax: ++49-351-458-4352


  • Author contributions: M.D.B.: conception and design, collection and assembly of data, data analysis and interpretation, drafting and critical revision of manuscript, and fund raising; M.H.: collection and assembly of data and critical revision of manuscript; A.S.: conception and design, data analysis and interpretation, drafting and critical revision of manuscript, and fund raising.

  • Disclosure of potential conflicts of interest is found at the end of this article.

  • §

    First published online in STEM CELLSEXPRESS September 17, 2012.

Abstract

Cell cycle analyses of adult hippocampal neural stem and precursor cells in vivo are challenging, as there is no temporal or local discrimination of different precursor cell populations. All commonly used techniques to determine the cell cycle length of proliferating cells in the adult hippocampus do not allow discrimination between different cell types. Here, we introduce a novel procedure to precisely calculate cell cycle phase lengths of distinct precursor cell populations in vivo and thereby demonstrate a large heterogeneity of cell cycle kinetics within the pool of adult hippocampal precursor cells. Proliferating NeuroD1+ cells exhibited a significantly faster S-phase progression (Ts = 10.1 ± 0.6 hours) and shorter total cell cycle length (Tc = 22.6 ± 0.1 hours) than NeuroD1 cells (Ts = 13.5 ± 0.8 hours, Tc = 27.0 ± 0.5 hours; p < .05). Dividing glial fibrillary acidic protein (GFAP+) cells also showed significantly shorter mean Ts of 9.7 ± 0.6 hours and Tc of 22.8 ± 0.5 hours compared to the rest of uncommitted NeuroD1 precursors (p < .01). Together, NeuroD1+ neuronal progenitors and mitotic GFAP+ radial glia-like cells divide significantly faster than amplifying neural progenitor cells by accelerating their S-phase. S-phase duration seems to determine cell cycle length in the adult hippocampus. STEM CELLS 2012;30:2843–2847

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