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Tissue-Specific Stem Cells
Article first published online: 27 NOV 2012
Copyright © 2012 AlphaMed Press
Volume 30, Issue 12, pages 2843–2847, December 2012
How to Cite
Brandt, M. D., Hübner, M. and Storch, A. (2012), Brief Report: Adult Hippocampal Precursor Cells Shorten S-Phase and Total Cell Cycle Length During Neuronal Differentiation. STEM CELLS, 30: 2843–2847. doi: 10.1002/stem.1244
Author contributions: M.D.B.: conception and design, collection and assembly of data, data analysis and interpretation, drafting and critical revision of manuscript, and fund raising; M.H.: collection and assembly of data and critical revision of manuscript; A.S.: conception and design, data analysis and interpretation, drafting and critical revision of manuscript, and fund raising.
Disclosure of potential conflicts of interest is found at the end of this article.
First published online in STEM CELLSEXPRESS September 17, 2012.
- Issue published online: 27 NOV 2012
- Article first published online: 27 NOV 2012
- Accepted manuscript online: 17 SEP 2012 03:52PM EST
- Manuscript Accepted: 11 JUL 2012
- Manuscript Received: 5 FEB 2012
- Deutsche Forschungsgemeinschaft (DFG). Grant Number: SFB655
- “From cells to tissues”. Grant Number: project A23
- Research Centre/Cluster of Excellence “Center for Regenerative Therapies Dresden (CRTD)”
- MeDDrive of the Medical Faculty Carl Gustav Carus at Dresden University of Technology
- Adult neurogenesis;
- Cell cycle kinetics;
Cell cycle analyses of adult hippocampal neural stem and precursor cells in vivo are challenging, as there is no temporal or local discrimination of different precursor cell populations. All commonly used techniques to determine the cell cycle length of proliferating cells in the adult hippocampus do not allow discrimination between different cell types. Here, we introduce a novel procedure to precisely calculate cell cycle phase lengths of distinct precursor cell populations in vivo and thereby demonstrate a large heterogeneity of cell cycle kinetics within the pool of adult hippocampal precursor cells. Proliferating NeuroD1+ cells exhibited a significantly faster S-phase progression (Ts = 10.1 ± 0.6 hours) and shorter total cell cycle length (Tc = 22.6 ± 0.1 hours) than NeuroD1− cells (Ts = 13.5 ± 0.8 hours, Tc = 27.0 ± 0.5 hours; p < .05). Dividing glial fibrillary acidic protein (GFAP+) cells also showed significantly shorter mean Ts of 9.7 ± 0.6 hours and Tc of 22.8 ± 0.5 hours compared to the rest of uncommitted NeuroD1− precursors (p < .01). Together, NeuroD1+ neuronal progenitors and mitotic GFAP+ radial glia-like cells divide significantly faster than amplifying neural progenitor cells by accelerating their S-phase. S-phase duration seems to determine cell cycle length in the adult hippocampus. STEM CELLS 2012;30:2843–2847