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Embryonic Stem Cells/Induced Pluripotent Stem Cells
Article first published online: 27 NOV 2012
Copyright © 2012 AlphaMed Press
Volume 30, Issue 12, pages 2709–2719, December 2012
How to Cite
Quarto, N., Li, S., Renda, A. and Longaker, M. T. (2012), Exogenous Activation of BMP-2 Signaling Overcomes TGFβ-Mediated Inhibition of Osteogenesis in Marfan Embryonic Stem Cells and Marfan Patient-Specific Induced Pluripotent Stem Cells. STEM CELLS, 30: 2709–2719. doi: 10.1002/stem.1250
Author contributions: Quarto N, conception and design, collection and/or assembly of data, data analysis and interpretation, manuscript writing, final approval of manuscript, Li S.: collection of data, renda A.: final approval of manuscript, administrative support, longaker M.: Financial support, Final approval of manuscript.
Disclosure of potential conflicts of interest is found at the end of this article.
First published online in STEM CELLSEXPRESS October 4, 2012.
- Issue published online: 27 NOV 2012
- Article first published online: 27 NOV 2012
- Accepted manuscript online: 4 OCT 2012 08:42AM EST
- Manuscript Accepted: 23 AUG 2012
- Manuscript Received: 12 JUN 2012
- National Institutes of Health. Grant Numbers: NIH-U01 HL099776, RC1 HL100490, RC2 DE020771
- California Institute Regenerative Medicine. Grant Number: #RL1-00662-1
Additional Supporting Information may be found in the online version of this article.
|sc-12-0555_sm_SupplFigure1.tif||295K||Supplemental Figure 1. Endogenous levels of CYCLIN D1 and CYCLIN E proteins in MFS and MFSiPS cells. Immunoblotting analysis using anti-CNND1 and anti-CNNE antibodies show higher amounts of endogenous CYCLIN D1 and CYCLIN E proteins in MFS and MFSiPS cells compared to their controls. Membranes were stripped and reprobed with β.ACTIN antibody as loading control. Histograms below represent quantification of CYCLIN D1 and CYCLIN E proteins obtained by Image J program.|
|sc-12-0555_sm_SupplFigure2.tif||2963K||Supplemental Figure 2. A, Expression profile of BMP ligands and their receptors. qPCR analysis of BMP-2, BMP-4 and BMPRIA and BMPRIB receptors showing similar levels of expression between MFS, MFFiPS cells and their corresponding WT controls. The relative mRNA level in each sample was normalized to its GAPDH content. Values are given as relative to GAPDH expression. B, ELISA assay does not detect significant differences of BMP-2 and -4 ligands between MFS, MFSiPS cells and their WT controls.|
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