Embryonic Stem Cells/Induced Pluripotent Stem Cells

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Concise Review: The Evolution of human pluripotent stem cell culture: From feeder cells to synthetic coatings†‡§

  1. L.G. Villa-Diaz1,
  2. A.M. Ross2,
  3. J. Lahann2,3,
  4. P.H. Krebsbach1,3,¶,*

Article first published online: 19 DEC 2012

DOI: 10.1002/stem.1260

Issue

STEM CELLS

STEM CELLS

Volume 31, Issue 1, pages 1–7, January 2013

Additional Information

How to Cite

Villa-Diaz, L.G., Ross, A.M., Lahann, J. and Krebsbach, P.H. (2013), Concise Review: The Evolution of human pluripotent stem cell culture: From feeder cells to synthetic coatings. STEM CELLS, 31: 1–7. doi: 10.1002/stem.1260

Author Information

  1. 1

    Department of Biologic & Materials Sciences, School of Dentistry

  2. 2

    Department of Chemical Engineering

  3. 3

    Department of Biomedical Engineering, University of Michigan, Ann Arbor, Michigan, USA

  1. PH: (734)-936-2600; Fax: (734)-647-2110

*Department of Biologic and Materials Sciences, University of Michigan School of Dentistry, 1011 North University Ave, Ann Arbor, Michigan 48109-1078, USA

  1. †

    Author contributions: L.G.V.-D., A.M.R., J.L., and P.H.K.: contributed in the preparation of this concise review.

  2. ‡

    Disclosure of potential conflicts of interest is found at the end of this article.

  3. §

    First published online in STEM CELLSEXPRESS October 18, 2012.

  4. PH: (734)-936-2600; Fax: (734)-647-2110

Publication History

  1. Issue published online: 19 DEC 2012
  2. Article first published online: 19 DEC 2012
  3. Accepted manuscript online: 18 OCT 2012 07:32AM EST
  4. Manuscript Accepted: 6 OCT 2012
  5. Manuscript Received: 5 AUG 2012

Funded by

  • NIH. Grant Number: DE016530

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Keywords:

  • Pluripotent stem cells;
  • Human embryonic stem cells;
  • Induced pluripotent stem cells;
  • Polymer coatings;
  • Self-renewal;
  • Differentiation

Abstract

Current practices to maintain human pluripotent stem cells (hPSCs), which include induced pluripotent stem cells and embryonic stem cells, in an undifferentiated state typically depend on the support of feeder cells such as mouse embryonic fibroblasts (MEFs) or an extracellular matrix such as Matrigel. Culture conditions that depend on these undefined support systems limit our ability to interpret mechanistic studies aimed at resolving how hPSCs interact with their extracellular environment to remain in a unique undifferentiated state and to make fate-changing lineage decisions. Likewise, the xenogeneic components of MEFs and Matrigel ultimately hinder our ability to use pluripotent stem cells to treat debilitating human diseases. Many of these obstacles have been overcome by the development of synthetic coatings and bioreactors that support hPSC expansion and self-renewal within defined culture conditions that are free from xenogeneic contamination. The establishment of defined culture conditions and synthetic matrices will facilitate studies to more precisely probe the molecular basis of pluripotent stem cell self-renewal and differentiation. When combined with three-dimensional cultures in bioreactors, these systems will also enable large-scale expansion for future clinical applications. STEM Cells2013;31:1–7

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