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Embryonic Stem Cells/Induced Pluripotent Stem Cells
Article first published online: 19 DEC 2012
Copyright © 2012 AlphaMed Press
Volume 31, Issue 1, pages 1–7, January 2013
How to Cite
Villa-Diaz, L.G., Ross, A.M., Lahann, J. and Krebsbach, P.H. (2013), Concise Review: The Evolution of human pluripotent stem cell culture: From feeder cells to synthetic coatings. STEM CELLS, 31: 1–7. doi: 10.1002/stem.1260
Author contributions: L.G.V.-D., A.M.R., J.L., and P.H.K.: contributed in the preparation of this concise review.
Disclosure of potential conflicts of interest is found at the end of this article.
First published online in STEM CELLSEXPRESS October 18, 2012.
- Issue published online: 19 DEC 2012
- Article first published online: 19 DEC 2012
- Accepted manuscript online: 18 OCT 2012 07:32AM EST
- Manuscript Accepted: 6 OCT 2012
- Manuscript Received: 5 AUG 2012
- NIH. Grant Number: DE016530
- Pluripotent stem cells;
- Human embryonic stem cells;
- Induced pluripotent stem cells;
- Polymer coatings;
Current practices to maintain human pluripotent stem cells (hPSCs), which include induced pluripotent stem cells and embryonic stem cells, in an undifferentiated state typically depend on the support of feeder cells such as mouse embryonic fibroblasts (MEFs) or an extracellular matrix such as Matrigel. Culture conditions that depend on these undefined support systems limit our ability to interpret mechanistic studies aimed at resolving how hPSCs interact with their extracellular environment to remain in a unique undifferentiated state and to make fate-changing lineage decisions. Likewise, the xenogeneic components of MEFs and Matrigel ultimately hinder our ability to use pluripotent stem cells to treat debilitating human diseases. Many of these obstacles have been overcome by the development of synthetic coatings and bioreactors that support hPSC expansion and self-renewal within defined culture conditions that are free from xenogeneic contamination. The establishment of defined culture conditions and synthetic matrices will facilitate studies to more precisely probe the molecular basis of pluripotent stem cell self-renewal and differentiation. When combined with three-dimensional cultures in bioreactors, these systems will also enable large-scale expansion for future clinical applications. STEM Cells2013;31:1–7