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Tissue-Specific Stem Cells
Article first published online: 25 FEB 2013
Copyright © 2012 AlphaMed Press
Volume 31, Issue 3, pages 511–525, March 2013
How to Cite
Cho, J. S., Kook, S. H., Robinson, A. R., Niedernhofer, L. J. and Lee, B.-C. (2013), Cell Autonomous and Nonautonomous Mechanisms Drive Hematopoietic Stem/progenitor Cell Loss in the Absence of DNA Repair. STEM CELLS, 31: 511–525. doi: 10.1002/stem.1261
Author contributions: J.S.C., S.H.K., and A.R.R.: collection and/or assembly of data, data analysis and interpretation; L.J.N. and B.L: data analysis and interpretation, conception and design, manuscript writing, and final approval of manuscript provision. J.S.C. and S.H.K. contributed equally to this work.
Disclosure of potential conflicts of interest is found at the end of this article.
First published online in STEM CELLSEXPRESS October 23, 2012.
- Issue published online: 25 FEB 2013
- Article first published online: 25 FEB 2013
- Accepted manuscript online: 23 OCT 2012 06:43AM EST
- Manuscript Accepted: 23 SEP 2012
- Manuscript Received: 6 JUL 2012
- Department of Defense. Grant Number: W81XWH-09-1-0364
- NIH. Grant Number: ES016114
- UPCI. Grant Number: P30CA047904
Additional Supporting Information may be found in the online version of this article.
|SC-12-0631_sm_SupplFigure1.pdf||35K||S1. Analysis of peripheral blood from 8-9 weeks and 21-24 weeks old Ercc1-/Δ mice and littermate controls. Units are WBC, RBC, and number of lymphocytes, monocytes and granulocytes: 103/mm; HGB and MCHC: g/dL; HCT and % lymphocytes, monocytes and granulocytes: percent; MCH: pictograms; MCV and MPV: femtoliters. * indicates statistical difference between genotypes, paired Student's t-test (8-9 weeks-old (n=7): MCV p=0.02; RDW p=0.0002; 21-24 weeks-old (n=5): WBC p=0.01; MCV p=0.003; # lymphocytes p=0.05).|
|SC-12-0631_sm_SupplFigure2.pdf||112K||S2. Bone marrow cells from donor mice (CD45.1, B6) were transplanted into lethally irradiated (10 Gy) wild type (Ercc1+/+) recipient mice (B6; FVB, CD45. 1/2). At 9 weeks posttransplantion, the recipients' peripheral blood (PB, upper panels) and bone marrow (BM, lower panels) were analyzed for donor cell contribution. Donor-derived bone marrow cells (CD45.1) in the recipient's bone marrow (n>3/each group) were gated and further analyzed for the frequency of LSK cells (CD45.1, LSK).|
|SC-12-0631_sm_SupplFigure3.pdf||166K||S3. LSK cells from 16- (n=3) and 21 week (n=6) old mice of the indicated genotypes were stained with anti-CXCR4 antibody. Upper: Shown are representative flow cytometry data. Numbers represent the percent of cells in each quadrant. Lower: Percentage of CXCR-4 positive LSK cells are shown as mean ± standard deviation (s.d.).|
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