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Tissue Specific Stem Cells
Article first published online: 19 DEC 2012
Copyright © 2012 AlphaMed Press
Volume 31, Issue 1, pages 215–220, January 2013
How to Cite
Yannarelli, G., Pacienza, N., Cuniberti, L., Medin, J., Davies, J. and Keating, A. (2013), Brief Report: The Potential Role of Epigenetics on Multipotent Cell Differentiation Capacity of Mesenchymal Stromal Cells. STEM CELLS, 31: 215–220. doi: 10.1002/stem.1262
Author contributions: G.Y.: conception and design, collection and/or assembly of data, data analysis and interpretation, manuscript writing, and final approval of manuscript; N.P., L.C., J.M., and J.E.D.: collection and/or assembly of data and data analysis and interpretation; A.K.: conception and design, manuscript writing, financial support, and final approval of manuscript.
Disclosure of potential conflicts of interest is found at the end of this article.
First published online in STEM CELLSEXPRESS October 23, 2012.
- Issue published online: 19 DEC 2012
- Article first published online: 19 DEC 2012
- Accepted manuscript online: 23 OCT 2012 06:43AM EST
- Manuscript Accepted: 4 OCT 2012
- Manuscript Received: 26 JUN 2012
- Orsino Translational Research Laboratory, Princess Margaret Hospital
- Gloria and Seymour Epstein Chair in Cell Therapy and Transplantation at University Health Network and the University of Toronto
- Mesenchymal stromal cells;
- Pluripotency factors;
Human umbilical cord perivascular cells (HUCPVCs) are a readily available source of mesenchymal stromal cells (MSCs) for cell therapy. We were interested in understanding how differences from human bone marrow (BM)-derived MSCs might yield insights into MSC biology. We found that HUCPVCs exhibited increased telomerase activity and longer telomeres compared with BM-MSCs. We also observed enhanced expression of the pluripotency factors OCT4, SOX2, and NANOG in HUCPVCs. The methylation of OCT4 and NANOG promoters was similar in both cell types, indicating that differences in the expression of pluripotency factors between the MSCs were not associated with epigenetic changes. MSC methylation at these loci is greater than reported for embryonic stem cells but less than in dermal fibroblasts, suggesting that multipotentiality of MSCs is epigenetically restricted. These results are consistent with the notion that the MSC population (whether BM- or HUCPV-derived) exhibits higher proliferative capacity and contains more progenitor cells than do dermal fibroblasts. STEM Cells2013;31:215–220