Additional Supporting Information may be found in the online version of this article.

sc-12-0799_sm_SupplFigure1.pdf105KSupplemental Figure 1: Keratin 14-Cre is active in the skin and throughout the primitive ductal tree by embryoni cday e18. X-gal stained K14-Cre+/−; R26R-/fl e18 mammary gland fat pad and adjoining skin. K14-Cre is active throughout the tree and within the skin where the gland branches out from the nipple (arrow).
sc-12-0799_sm_SupplFigure2.pdf1805KSupplemental Figure 2: K14-Cre is active throughout the developing mammary epithelium throughout branching morphogenesis into adulthood. X-gal stained FGFR1+/fl; K14-Cre+/−; R26R-/fl and FGFR1fl/fl; K14-Cre+/−; R26R-/fl glands at 5, 7 and 12 weeks of development.
sc-12-0799_sm_SupplFigure3.pdf75KSupplemental Figure 3: Both of FGFR1 and R2 are expressed in the basal and luminal cell subpopulations. A. FACS profile of luminal CD24+CD49flo and basal CD24+CD49fhi sorted populations. B. qPCR for the luminal marker, Keratin 8, and basal marker, Keratin 14, shows proper segregation and enrichment of the luminal and basal epithelial populations after sorting. Keratin 8 is enriched in the luminal compartment, while Keratin 14 is enriched in the basal compartment. C. qPCR analysis of FGFR1 and FGFR2 reveals expression of each receptor in both compartments, with enrichment of both receptors in the luminal compartment.
sc-12-0799_sm_SupplTable1.pdf31KSupplemental Table 1: List of antibodies used for all methods including vendor and catalog number.

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