Embryonic Stem Cells/Induced Pluripotent Stem Cells
Version of Record online: 12 FEB 2013
Copyright © 2012 AlphaMed Press
Volume 31, Issue 2, pages 408–414, February 2013
How to Cite
Boucherie, C., Mukherjee, S., Henckaerts, E., Thrasher, A. J., Sowden, J. C. and Ali, R. R. (2013), Brief Report: Self-Organizing Neuroepithelium from Human Pluripotent Stem Cells Facilitates Derivation of Photoreceptors. STEM CELLS, 31: 408–414. doi: 10.1002/stem.1268
Author contributions: C.B.: conception and design, collection and/or assembly of data, data analysis and interpretation, and manuscript writing; S.M.: provision of study material and collection and/or assembly of data; E.H. and A.J.T.: provision of study material; J.C.S.: financial support, data analysis and interpretation, and manuscript writing; R.R.A.: financial support, administrative support, data analysis and interpretation, manuscript writing, and final approval of manuscript.
Disclosure of potential conflicts of interest is found at the end of this article.
First published online in STEM CELLSEXPRESS November 6, 2012.
- Issue online: 12 FEB 2013
- Version of Record online: 12 FEB 2013
- Accepted manuscript online: 6 NOV 2012 11:32PM EST
- Manuscript Accepted: 11 OCT 2012
- Manuscript Received: 27 AUG 2012
- Medical Research Council U.K.. Grant Numbers: G03000341, MR/J004553/1
- The Millers Trust
- Great Ormond Street Hospital Children's Charity
- Department of Health's National Institute for Health Research Biomedical Research Centre at Moorfields Eye Hospital
Additional Supporting Information may be found in the online version of this article.
|sc-12-0797_sm_SupplFigure1.pdf||74K||Figure S 1. Neuroepithelium formed in ECM displays luminal cell mitosis (A-C) Accumulation of F-actin at the apical side of the neuroepithelium formed after 2 days of differentiation illustrates its polarization. Cells in division (pH3+) are detected only at the apical side of the neuroepithelium. Abbreviation: as; apical side, bs; basal side. Scale bars: 50 μm (A) and 10 μm (B, C).|
|sc-12-0797_sm_SupplFigure2.pdf||55K||Figure S2. Rapid induction of retinal progenitor specific transcripts using ECM Time course gene expression study revealed that PAX6 and RAX transcripts are expressed from day 2.|
|sc-12-0797_sm_SupplFigure3.pdf||84K||Figure S3. Cell death is not implicated in the down regulation of cone markers (A-D) An antibody specific to the active form of Caspase-3 to detect apoptotic cells illustrates that cell death is a marginal process between day 6 and 10 of differentiation. Scale bars: 50 μm (A-D).|
|sc-12-0797_sm_SupplFigure4.pdf||97K||Figure S4. Characterization of hiPS cells (A-D) Overexpression of Oct4, Sox2, c-Myc and Klf4 reprogrammed human fibroblasts into hiPS cells with typical hES cells morphology which expressed SSEA-4, TRA-1-81 and TRA-1- 60. In addition, these cells expressed oct4, nanog and sox2 (E) and formed embryoid bodies expressing markers of neuroectoderm, mesoderm and endoderm (F). Scale bars: 100 μm (A) and 20 μm (B-D).|
|sc-12-0797_sm_SupplMovie1.mov||5389K||Movie S1. Time-lapse imaging of hES cell clusters in Matrigel forming a neural epithelium over 48 hours.|
|sc-12-0797_sm_SupplTable1.pdf||75K||Table S1. Primers used in this study.|
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