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Additional Supporting Information may be found in the online version of this article.

FilenameFormatSizeDescription
sc-12-0797_sm_SupplFigure1.pdf74KFigure S 1. Neuroepithelium formed in ECM displays luminal cell mitosis (A-C) Accumulation of F-actin at the apical side of the neuroepithelium formed after 2 days of differentiation illustrates its polarization. Cells in division (pH3+) are detected only at the apical side of the neuroepithelium. Abbreviation: as; apical side, bs; basal side. Scale bars: 50 μm (A) and 10 μm (B, C).
sc-12-0797_sm_SupplFigure2.pdf55KFigure S2. Rapid induction of retinal progenitor specific transcripts using ECM Time course gene expression study revealed that PAX6 and RAX transcripts are expressed from day 2.
sc-12-0797_sm_SupplFigure3.pdf84KFigure S3. Cell death is not implicated in the down regulation of cone markers (A-D) An antibody specific to the active form of Caspase-3 to detect apoptotic cells illustrates that cell death is a marginal process between day 6 and 10 of differentiation. Scale bars: 50 μm (A-D).
sc-12-0797_sm_SupplFigure4.pdf97KFigure S4. Characterization of hiPS cells (A-D) Overexpression of Oct4, Sox2, c-Myc and Klf4 reprogrammed human fibroblasts into hiPS cells with typical hES cells morphology which expressed SSEA-4, TRA-1-81 and TRA-1- 60. In addition, these cells expressed oct4, nanog and sox2 (E) and formed embryoid bodies expressing markers of neuroectoderm, mesoderm and endoderm (F). Scale bars: 100 μm (A) and 20 μm (B-D).
sc-12-0797_sm_SupplMovie1.mov5389KMovie S1. Time-lapse imaging of hES cell clusters in Matrigel forming a neural epithelium over 48 hours.
sc-12-0797_sm_SupplTable1.pdf75KTable S1. Primers used in this study.

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