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Cancer Stem Cells
Article first published online: 19 DEC 2012
Copyright © 2012 AlphaMed Press
Volume 31, Issue 1, pages 23–34, January 2013
How to Cite
Yuan, S., Wang, F., Chen, G., Zhang, H., Feng, L., Wang, L., Colman, H., Keating, M. J., Li, X., Xu, R.-H., Wang, J. and Huang, P. (2013), Effective Elimination of Cancer Stem Cells By a Novel Drug Combination Strategy. STEM CELLS, 31: 23–34. doi: 10.1002/stem.1273
Author contributions: S.Y. and F.W.: conception and design, collection/assembly of data, data analysis/interpretation, manuscript writing, and final approval; G.C., H.Z., L.W., H.C., M.K., R.X., and J.W.: provision of study materials and final approval; L.F.: collection/assembly of data; X.L.: design of animal study and data analysis/interpretation; P.H.: conception and design, provision of study materials, data analysis/interpretation, and final approval; S.Y. and F.W. contributed equally to this article.
Disclosure of potential conflicts of interest is found at the end of this article.
First published online in STEM CELLSEXPRESS November 6, 2012.
- Issue published online: 19 DEC 2012
- Article first published online: 19 DEC 2012
- Accepted manuscript online: 6 NOV 2012 11:33PM EST
- Manuscript Accepted: 29 SEP 2012
- Manuscript Received: 1 MAY 2012
- major science and technology project
- National Basic Research Program (973 Program) of China. Grant Numbers: 2012CB967004, CA085563, CA100428, CA16672
- National Institutes of Health
- CLL Global Research Foundation
- Rosalie B Hite Fellowship from MD Anderson Cancer Center
Additional Supporting Information may be found in the online version of this article.
|sc-12-0416_sm_SupplFigure1.tif||2868K||Figure S1. Cytotoxic effect of 3-BrOP and BCNU on serum-induced GSC23 cells. The glioma stem cells (GSC23) were first induced to undergo differentiation by exposure to serum (10% FBS, 5 days). The effect of 3-BrOP, BCNU, or their combination on the serum-induced cells was then tested under hypoxia (2% oxygen for 24 h). Cell viability was measured by annexin-V/PI double-staining followed by flow cytometry analysis. The number (%) in each panel shows the percent of viable cells (annexin-V/PI double-negative).|
|sc-12-0416_sm_SupplFigure2.tif||2362K||Figure S2. Proliferation of GSC11, CSC23, U87, and NHA cells. (A) Cell growth curves of GSC11, serum-induced GSC11, GSC23, and serum-induced GSC23 cells. (B) Doubling time of GSC11, serum-induced GSC11, GSC23, serum-induced GSC23, U87 cells, and normal human astrocytes (NHA).|
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