Additional Supporting Information may be found in the online version of this article.

sc-12-0416_sm_SupplFigure1.tif2868KFigure S1. Cytotoxic effect of 3-BrOP and BCNU on serum-induced GSC23 cells. The glioma stem cells (GSC23) were first induced to undergo differentiation by exposure to serum (10% FBS, 5 days). The effect of 3-BrOP, BCNU, or their combination on the serum-induced cells was then tested under hypoxia (2% oxygen for 24 h). Cell viability was measured by annexin-V/PI double-staining followed by flow cytometry analysis. The number (%) in each panel shows the percent of viable cells (annexin-V/PI double-negative).
sc-12-0416_sm_SupplFigure2.tif2362KFigure S2. Proliferation of GSC11, CSC23, U87, and NHA cells. (A) Cell growth curves of GSC11, serum-induced GSC11, GSC23, and serum-induced GSC23 cells. (B) Doubling time of GSC11, serum-induced GSC11, GSC23, serum-induced GSC23, U87 cells, and normal human astrocytes (NHA).

Please note: Wiley Blackwell is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.