Additional Supporting Information may be found in the online version of this article.

sc-12-0345_sm_SupplData.pdf17KSupplementary Data
sc-12-0345_sm_SupplTable1.pdf8KSupplementary Table 1
sc-12-0345_sm_SupplFigure1.tif2946KSupplemental Figure 1. Characterization of bone marrow hematopoietic stem cells. Further characterization of tc-ptp+/+ Lin−CD117+Sca-1+CD105+ and Lin−CD117+Sca-1+CD105- bone marrow stem cells subpopulations by flow cytometry showing expression of CD150, Flk-2 and CD34.
sc-12-0345_sm_SupplFigure2.tif901KSupplemental Figure 2. Assessment of apoptosis in hematopoietic stem cells. tc-ptp+/+ and tc-ptp−/− bone marrow (BM) were stained for hematopoietic stem cells as well as Annexin V and propidium iodide (PI) for analyzed by flow cytometry. The results shown are represented as Annexin V and PI and are gated on BM hematopoietic stem cells (HSC), which were identified as Lin-CD117+Sca-1+CD105+ cells.
sc-12-0345_sm_SupplFigure3.tif858KSupplemental Figure 3. Inhibition of tc-ptp by RNAi treatment. Mouse and human hematopoietic stem cells were electroporated without RNAi (PBS), with a control scramble sequence (SCR), or with TC-PTP RNAi (TC1 and/or TC2). RNA was isolated 72h post treatment from (A) Lin−CD117+Sca-1+CD105+ hematopoietic stem cells from the bone marrow of Balb/c mice and (B) Lin-CD34+CD133+ hematopoietic stem cells from human bone marrow. RT-PCR was performed to analyze levels of TC-PTP. GAPDH was used as a loading control.
sc-12-0345_sm_SupplFigure4.tif1118KSupplemental Figure 4. Further increased cytokine signaling in tc-ptp−/− Lin−CD117+Sca-1hi CD105+ subpopulation. Flow cytometry analysis of bone marrow Lin−CD117+Sca-1+ CD105+ cells from tc-ptp+/+ mice (thin line), tc-ptp−/− Lin−CD117+Sca-1+/hiCD105+ (thick line) and from tc-ptp−/− Lin−CD117+Sca-1hiCD105+ (extra-thick line) was performed to assess intracellular expression of (A) IL-18, (B) IL-18 binding protein (IL-18bp), or (C) phosphorylated Stat1 (p- Stat1) and total Stat1 protein. To better compare subpopulations, both the thin and thick line in all histograms have been reproduced as shown in Figure 5. A further overlay of the tc-ptp−/− Lin− CD117+Sca-1hiCD105+ (extra-thick line) population was performed. Nonspecific IgG was used as negative control (A, B, C: first flow cytometry histogram). The relative cell number, expressed as % max, is plotted against IgG, IL-18, IL-18bp, p-Stat1 or Stat1 fluorescence to compensate for the difference in cellularity between the populations. The mean fluorescence intensity (MFI) is indicated in the corresponding plot.

Please note: Wiley Blackwell is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.