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Additional Supporting Information may be found in the online version of this article.

FilenameFormatSizeDescription
sc-12-0607_sm_SupplData.pdf33KSupplementary Data
sc-12-0607_sm_SupplFigure1.tif1027KFigure S1. Algorithm for correlating miRNAs with their target mRNAs in pluripotent cells.
sc-12-0607_sm_SupplFigure2.tif2545KFigure S2. (A) The miR-302 inhibits NR2F2 3′UTR reporter activity. The lentiviral miR- 302a/b/c/d (Lv-302) suppresses the activity of T-WT reporter. However, mutations in either binding site reduce this suppression. The reporter with double 3′UTR mutations showed luciferase activity similar to control (pGL3-Con). (B) The miR-367 can not inhibit NR2F2 3′UTR reporter activity.
sc-12-0607_sm_SupplFigure3.pdf106KFigure S3. Electroporation of GFP reporter into H7 hESCs. The hESCs were electroporated with GFP reporter using the AmaxaR Human Stem Cell NucleofectorR Kit II. With electroporation, typical transfection efficiency is estimated at 80%. Scale bar=50 μm.
sc-12-0607_sm_SupplFigure4.tif829KFigure S4. Knockdown of NR2F2. The shRNA against NR2F2 led to approximately 50% knockdown of endogenous NR2F2 in HeLa cells.
sc-12-0607_sm_SupplFigure5.tif1294KFigure S5. The expression level of both miR-302b and NR2F2 upon the reprogramming initiation. (A) The reprogramming with the addition of Lv-shNR2F2 or Lv-NR2F2. (B) The reprogramming with Lv-302 and/or Lv-NR2F2.
sc-12-0607_sm_SupplFigure6.pdf274KFigure S6. Typical colonies used for the evaluation of reprogramming efficiency. These colonies were AKP+, TRA-1-60+, and had hESC-like morphology. Note that colonies that lacked TRA-1-60 and/or did not have hESC-like morphology were discarded from our analysis. Scale bar=50μm.
sc-12-0607_sm_SupplFigure7.pdf381KFigure S7. Representative hematoxylin and eosin staining of a solid encapsulated teratoma derived from typical iPSC lines implanted into the sub-renal capsule of SCID mice. Histological analysis of tissue demonstrated presence of glandular structures (endoderm), cartilage (mesoderm), and neural rosettes (ectoderm). Scale bar=250 μm.
sc-12-0607_sm_SupplFigure8.pdf280KFigure S8. Characterization of KMOS and KMOS3-iPSC-derived endothelial cells (ECs). The differentiated and purified endothelial cells expressed typical EC markers such as CD31 and CD144. Furthermore, these endothelial cells showed tube formation on Matrigel and low-density lipoprotein uptake, indicating that they are functional endothelial cells. Scale bar=50 μm.
sc-12-0607_sm_SupplFigure9.pdf183KFigure S9. Characterization of KMOS- and KMOS3-iPSC-derived cardiomyocytes. The differentiated cardiomyocytes expressed cardiac-specific TNNT2 and transcription factor MEF2C. Scale bar=50 μm.
sc-12-0607_sm_SupplFigure10.pdf156KFigure S10. Characterization of iPSC-derived neural stem cells (NSCs). The differentiated NSCs expressed NESTIN and transcription factor SOX2. Scale bar=50 μm.
sc-12-0607_sm_SupplFigure11.tif1440KFigure S11. Schematic diagram showing predicted miR-302b induced regulatory circuit in pluripotent cells.
sc-12-0607_sm_SupplFigure12.tif1535KFigure S12. OCT4, miR-302, and NR2F2 regulatory models in pluripotent cells and non- pluripotent cells based on experimental results from our study. Blue line represents clustering in pluripotent cells; black line represents clustering in differentiated cells.
sc-12-0607_sm_SupplMovie1.avi3536KMovie S1. After 14 days of cardiac differentiation of KMOS iPSCs, spontaneously contracting cell layers are observed under light microscopy.
sc-12-0607_sm_SupplMovie2.avi2033KMovie S2. After 14 days of cardiac differentiation of KMOS3 iPSCs, spontaneously contracting cell layers are observed under light microscopy.
sc-12-0607_sm_SupplTable1.pdf22KSupplementary Table 1
sc-12-0607_sm_SupplTable2.pdf26KSupplementary Table 2
sc-12-0607_sm_SupplTable3.pdf21KSupplementary Table 3

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