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Additional Supporting Information may be found in the online version of this article.

FilenameFormatSizeDescription
sc-12-0609_sm_SupplFigure1.tif1226KFigure S1. A customized SuperArray membrane was constructed with 111 selected genes based on genome-wide transcriptome analysis of mouse oocytes during in vitro maturation.
sc-12-0609_sm_SupplFigure2.tif551KFigure S2. mESCs were differentiated into mesoendoderm lineage for 4 days and markers representing mesoderm and endoderm were analyzed for the expression by real-time RT-PCR. All values are means ±;s.d. from at least triplicate experiments. * indicates significant (P<0.05) and ** highly significant (p<0.01) results based on ANOVA analyses following the Scheffe test.
sc-12-0609_sm_SupplFigure3.tif606KFigure S3. 4 weeks old teratoma of WT and Tcea3 KD were analyzed for the expression of markers representing each lineage by real-time RT-PCR. The expression level of each gene was shown as relative value following normalization against that of the glyceraldehyde 3-phosphate dehydrogenase (Gapdh) gene.
sc-12-0609_sm_SupplFigure4.tif938KFigure S4. Chromatin immunoprecipitation using antibodies against Tcea3 and rabbit IgG (as an immunoprecipitation control). DNA fragments were analyzed by PCR using primers specific for the proximal promoter region (Chip1) and downstream region of the Lefty1 gene (Chip2) (left). Relative quantification of Tcea3 immunoprecipitation enrichment at the Chip1 and Chip2 region was performed with WT, Tcea3 KD and Tcea3 OE cells. All values are means ±;s.d. from at least triplicate experiments. * indicates significant (P<0.05) results based on Student's T-test analyses.
sc-12-0609_sm_SupplFigure5.tif1796KFigure S5. (A) Immunoblot analysis of p-Smad1/5 in Tcea3 OE and WT mESCs. Total cell extracts were prepared either from undifferentiated cells or 4 days after RA-induced differentiation. β-actin was used as loading control. (B) Expression levels of BMP4, ID1, ID2 and ID3 by microarray analysis of Tcea3 OE cells. (C) WT and Tcea3 OE cells were serum starved for 24 hrs and stimulated with Nodal (1 μg/ml). Cells were harvested at the indicated times after Nodal stimulation and pSmad2 (left panel) or pSmad1/5 (right panel) were analyzed by immunoblot.
sc-12-0609_sm_SupplFigure6.tif62KFigure S6. Microarray analysis of Tcea3 OE shows mesoderm marker genes of Tcea3 OE are activated compared to wild type mESCs under self-renewing condition.
sc-12-0609_sm_SupplTable1.pdf55KTable S1. Selected candidates identified as binding proteins of Tcea3.
sc-12-0609_sm_SupplTable2.pdf60KTable S2. 359 genes whose expression was significantly up- or down- regulated in Tcea3 OE mESCs.
sc-12-0609_sm_SupplTable3.pdf11KTable S3. RT-PCR primers used in this study

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