Both should be considered as first author
Tissue-Specific Stem Cells
Article first published online: 12 FEB 2013
Copyright © 2012 AlphaMed Press
Volume 31, Issue 2, pages 384–396, February 2013
How to Cite
Saclier, M., Yacoub-Youssef, H., Mackey, A. L., Arnold, L., Ardjoune, H., Magnan, M., Sailhan, F., Chelly, J., Pavlath, G. K., Mounier, R., Kjaer, M. and Chazaud, B. (2013), Differentially Activated Macrophages Orchestrate Myogenic Precursor Cell Fate During Human Skeletal Muscle Regeneration. STEM CELLS, 31: 384–396. doi: 10.1002/stem.1288
Author contributions: M.S. and H.Y.-Y.: collection and/or assembly of data and data analysis and interpretation; A.L.M.: collection and/or assembly of data and manuscript writing; L.A., H.A., and M.M.: collection and/or assembly of data; F.S. and J.C.: provision of study material or patients; G.K.P.: conception and design and manuscript writing; R.M.: data analysis and interpretation and manuscript writing; M.K. and B.C.: conception and design, data analysis and interpretation, and manuscript writing. M.S. and H.Y.-Y. are first authors.
Disclosure of potential conflicts of interest is found at the end of this article.
First published online in STEM CELLSEXPRESS November 21, 2012.
- Issue published online: 12 FEB 2013
- Article first published online: 12 FEB 2013
- Accepted manuscript online: 21 NOV 2012 12:33AM EST
- Manuscript Accepted: 28 OCT 2012
- Manuscript Received: 11 JUN 2012
- European Community's Seventh Framework Programme. Grant Number: FP7-Health—2009 ENDOSTEM 241440
- Agence Nationale pour la Recherche (ANR)
- Association Française contre les Myopathies (AFM)
- Nordea Foundation (Healthy Aging grant)
- Danish Ministry of Health, and MYOAGE. Grant Number: 223576
Additional Supporting Information may be found in the online version of this article.
|sc-12-0535_SupplFigure1.tif||420K||Supplemental Figure 1. Flow cytometry analysis of MPCs and MPs. (A) Primary human MPCs were cultured and sorted before passage 1 with anti-CD56 magnetic beads and analysed by flow cytometry for CD56 expression (B). Human MPs were activated as M1 MPs, M2a MPs or M2c MPs and analysed for their expression of pan-macrophagic markers (CD14, HLA-DR) and markers associated with the pro-inflammatory state (CD80), alternative activation state (CD206) and anti- inflammatory state (CD163). Results are means ± s.e.m of 3 independent experiments.|
|sc-12-0535_SupplFigure2.tif||4582K||Supplemental Figure 2. Model of in vivo human muscle regeneration. Vastus lateralis muscle was subjected to voluntary lengthening contractions and electrostimulation. The contralateral leg was biopsied at day 0. Then the damaged leg was biopsied at day 7 and 30 after damage induction. Histological sections were stained for HE, and were immunolabelled for laminin, embryonic myosin heavy chain or CD56 (blue = Hoechst). Bar=50 μm. Pictures are representative of 3 independent experiments.|
|sc-12-0535_SupplFigure3.tif||310K||Supplemental Figure 3. In vivo costaining of CD68 and CD206. Sections from human muscle day 7 after electrostimulation were stained for CD68 (green) and CD206 (red) markers. Most of the CD206pos cells were also labelled for CD68, indicating that they were macrophages. Few cells outside the regenerating area were positive for CD206 alone and were likely myogenic or satellite cells (arrows). Bar=50 μm.|
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