Additional Supporting Information may be found in the online version of this article.

sc-12-0535_SupplFigure1.tif420KSupplemental Figure 1. Flow cytometry analysis of MPCs and MPs. (A) Primary human MPCs were cultured and sorted before passage 1 with anti-CD56 magnetic beads and analysed by flow cytometry for CD56 expression (B). Human MPs were activated as M1 MPs, M2a MPs or M2c MPs and analysed for their expression of pan-macrophagic markers (CD14, HLA-DR) and markers associated with the pro-inflammatory state (CD80), alternative activation state (CD206) and anti- inflammatory state (CD163). Results are means ± s.e.m of 3 independent experiments.
sc-12-0535_SupplFigure2.tif4582KSupplemental Figure 2. Model of in vivo human muscle regeneration. Vastus lateralis muscle was subjected to voluntary lengthening contractions and electrostimulation. The contralateral leg was biopsied at day 0. Then the damaged leg was biopsied at day 7 and 30 after damage induction. Histological sections were stained for HE, and were immunolabelled for laminin, embryonic myosin heavy chain or CD56 (blue = Hoechst). Bar=50 μm. Pictures are representative of 3 independent experiments.
sc-12-0535_SupplFigure3.tif310KSupplemental Figure 3. In vivo costaining of CD68 and CD206. Sections from human muscle day 7 after electrostimulation were stained for CD68 (green) and CD206 (red) markers. Most of the CD206pos cells were also labelled for CD68, indicating that they were macrophages. Few cells outside the regenerating area were positive for CD206 alone and were likely myogenic or satellite cells (arrows). Bar=50 μm.

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