Ceramide-1-Phosphate Regulates Migration of Multipotent Stromal Cells and Endothelial Progenitor Cells—Implications for Tissue Regeneration§

Authors

  • Chihwa Kim,

    1. Stem Cell Institute at the James Graham Brown Cancer Center, University of Louisville, Kentucky, USA
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  • Gabriela Schneider,

    1. Stem Cell Institute at the James Graham Brown Cancer Center, University of Louisville, Kentucky, USA
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  • Ahmed Abdel-Latif,

    1. Division of Cardiovascular Medicine, Gill Heart Institute, University of Kentucky, Lexington, Kentucky, USA
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  • Kasia Mierzejewska,

    1. Stem Cell Institute at the James Graham Brown Cancer Center, University of Louisville, Kentucky, USA
    2. Department of Physiology, Pomeranian Medical University, Szczecin, Poland
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  • Manjula Sunkara,

    1. Division of Cardiovascular Medicine, Gill Heart Institute, University of Kentucky, Lexington, Kentucky, USA
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  • Sylwia Borkowska,

    1. Stem Cell Institute at the James Graham Brown Cancer Center, University of Louisville, Kentucky, USA
    2. Department of Physiology, Pomeranian Medical University, Szczecin, Poland
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  • Janina Ratajczak,

    1. Stem Cell Institute at the James Graham Brown Cancer Center, University of Louisville, Kentucky, USA
    2. Department of Physiology, Pomeranian Medical University, Szczecin, Poland
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  • Andrew J. Morris,

    1. Division of Cardiovascular Medicine, Gill Heart Institute, University of Kentucky, Lexington, Kentucky, USA
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  • Magda Kucia,

    1. Stem Cell Institute at the James Graham Brown Cancer Center, University of Louisville, Kentucky, USA
    2. Department of Physiology, Pomeranian Medical University, Szczecin, Poland
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  • Mariusz Z. Ratajczak

    Corresponding author
    1. Stem Cell Institute at the James Graham Brown Cancer Center, University of Louisville, Kentucky, USA
    2. Department of Physiology, Pomeranian Medical University, Szczecin, Poland
    • Hoenig Endowed Chair, Professor and Director Stem Cell Institute at James Graham Brown Cancer Center, University of Louisville, 500 S. Floyd Street, Rm. 107, Louisville, Kentucky 40202, USA
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    • Author contributions: C.H.K., G.S., A.J.M., and A.A.-L.: collection and/or assembly of data and final approval of manuscript; K.M., J.R., and M.K.: collection and/or assembly of data; M.S. and S.B.: collection of data; M.Z.R.: conception and design, financial support, and manuscript writing.

    • Telephone: 502-852-1788; Fax: 502-852-3032


  • Disclosure of potential conflicts of interest is found at the end of this article.

  • §

    First published online in STEM CELLSEXPRESS November 29, 2012.

Abstract

Ceramide-1-phosphate (C1P) is a bioactive lipid that, in contrast to ceramide, is an antiapoptotic molecule released from cells that are damaged and “leaky.” As reported recently, C1P promotes migration of hematopoietic cells. In this article, we tested the hypothesis that C1P released upon tissue damage may play an underappreciated role in chemoattraction of various types of stem cells and endothelial cells involved in tissue/organ regeneration. We show for the first time that C1P is upregulated in damaged tissues and chemoattracts bone marrow (BM)-derived multipotent stromal cells, endothelial progenitor cells, and very small embryonic-like stem cells. Furthermore, compared to other bioactive lipids, C1P more potently chemoattracted human umbilical vein endothelial cells and stimulated tube formation by these cells. C1P also promoted in vivo vascularization of Matrigel implants and stimulated secretion of stromal cell-derived factor-1 from BM-derived fibroblasts. Thus, our data demonstrate, for the first time, that C1P is a potent bioactive lipid released from damaged cells that potentially plays an important and novel role in recruitment of stem/progenitor cells to damaged organs and may promote their vascularization. STEM CELLS2013;31:500–510

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