Author contributions: C.H.K., G.S., A.J.M., and A.A.-L.: collection and/or assembly of data and final approval of manuscript; K.M., J.R., and M.K.: collection and/or assembly of data; M.S. and S.B.: collection of data; M.Z.R.: conception and design, financial support, and manuscript writing.
Article first published online: 25 FEB 2013
Copyright © 2012 AlphaMed Press
Volume 31, Issue 3, pages 500–510, March 2013
How to Cite
Kim, C., Schneider, G., Abdel-Latif, A., Mierzejewska, K., Sunkara, M., Borkowska, S., Ratajczak, J., Morris, A. J., Kucia, M. and Ratajczak, M. Z. (2013), Ceramide-1-Phosphate Regulates Migration of Multipotent Stromal Cells and Endothelial Progenitor Cells—Implications for Tissue Regeneration. STEM CELLS, 31: 500–510. doi: 10.1002/stem.1291
Disclosure of potential conflicts of interest is found at the end of this article.
First published online in STEM CELLSEXPRESS November 29, 2012.
- Issue published online: 25 FEB 2013
- Article first published online: 25 FEB 2013
- Accepted manuscript online: 29 NOV 2012 05:49AM EST
- Manuscript Accepted: 10 NOV 2012
- Manuscript Revised: 8 NOV 2012
- Manuscript Received: 17 JUL 2012
- NIH. Grant Number: 2R01 DK074720
- Stella and Henry Endowment, the European Union structural
- Innovative Economy Operational Program. Grant Number: POIG.01.01.02-00-109/09-00
- Maestro. Grant Number: 2011/02/A/NZ4/00035
- Multipotent stromal cell;
- Human umbilical vein endothelial cell;
Ceramide-1-phosphate (C1P) is a bioactive lipid that, in contrast to ceramide, is an antiapoptotic molecule released from cells that are damaged and “leaky.” As reported recently, C1P promotes migration of hematopoietic cells. In this article, we tested the hypothesis that C1P released upon tissue damage may play an underappreciated role in chemoattraction of various types of stem cells and endothelial cells involved in tissue/organ regeneration. We show for the first time that C1P is upregulated in damaged tissues and chemoattracts bone marrow (BM)-derived multipotent stromal cells, endothelial progenitor cells, and very small embryonic-like stem cells. Furthermore, compared to other bioactive lipids, C1P more potently chemoattracted human umbilical vein endothelial cells and stimulated tube formation by these cells. C1P also promoted in vivo vascularization of Matrigel implants and stimulated secretion of stromal cell-derived factor-1 from BM-derived fibroblasts. Thus, our data demonstrate, for the first time, that C1P is a potent bioactive lipid released from damaged cells that potentially plays an important and novel role in recruitment of stem/progenitor cells to damaged organs and may promote their vascularization. STEM CELLS2013;31:500–510