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Version of Record online: 25 FEB 2013
Copyright © 2012 AlphaMed Press
Volume 31, Issue 3, pages 607–611, March 2013
How to Cite
Singh, L., Brennan, T. A., Kim, J.-H., Egan, K. P., Mcmillan, E. A., Chen, Q., Hankenson, K. D., Zhang, Y., Emerson, S. G., Johnson, F. B. and Pignolo, R. J. (2013), Brief Report: Long-Term Functional Engraftment of Mesenchymal Progenitor Cells in a Mouse Model of Accelerated Aging. STEM CELLS, 31: 607–611. doi: 10.1002/stem.1294
Author contributions: L.S., T.B., J.-H.K., and Y.Z.: collection and/or assembly of data, data analysis and interpretation, manuscript writing, and final approval of manuscript; K.P.E. and E.A.M.: collection and/or assembly of data and final approval of manuscript; Q.C.: collection and/or assembly of data, provision of study materials, and final approval of manuscript; K.D.H. and S.G.E.: data analysis and interpretation, manuscript writing, and final approval of manuscript; F.B.J.: collection and/or assembly of data, provision of study materials, data analysis and interpretation, manuscript writing, and final approval of manuscript; R.J.P.: conception and design, financial support, collection and/or assembly of data, data analysis and interpretation, manuscript writing, and final approval of manuscript. L.S. and T.A.B. contributed equally to this article.
Disclosure of potential conflicts of interest is found at the end of this article.
First published online in STEM CELLSEXPRESS November 29, 2012.
- Issue online: 25 FEB 2013
- Version of Record online: 25 FEB 2013
- Accepted manuscript online: 29 NOV 2012 05:50AM EST
- Manuscript Accepted: 5 NOV 2012
- Manuscript Revised: 26 OCT 2012
- Manuscript Received: 16 AUG 2012
- National Institutes of Health/National Institute. Grant Number: R01AG028873
- University of Pennsylvania Institute
- Penn Center for Musculoskeletal Disorders
Additional Supporting Information may be found in the online version of this article.
|sc-12-0760_sm_SupplFigure1.pdf||147K||Figure S1. (A) Adherent MPCs from young wild-type donors are CD45− Sca-1+ cells. Gated cells are shown in the left panel. Cells are > 99.5% CD45− and > 96.5% Sca-1+ (right panel). SSC, side scatter; FSC, forward scatter. (B) Differentiation of GFP+ CD45− Sca-1+ mesenchymal progenitor cells (MPCs) into osteoblasts and adipocytes. Original magnification, 200X.|
|sc-12-0760_sm_SupplFigure2.pdf||4401K||Figure S2. Donor MPCs differentiate into bone-lining osteoblasts (and subsequently osteocytes) that are incorporated into bone. Arrowheads indicate representative bone- lining cells. Arrows indicate representative osteocytes in their lacunae. Scale bar = 10 μm.|
|sc-12-0760_sm_SupplFigure3.pdf||75K||Figure S3. BMT in Wrn−/−Terc−/− animals preserves bone osteoblasts/bone surface (N. Ob/BS) and number of osteoclasts/bone surface (N. Oc/BS).|
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