Additional Supporting Information may be found in the online version of this article.

sc-12-0811_sm_SupplFigure1.pdf195KSupporting Information Figure S1. PyMT expression induced the formation of adenomas that progressed to carcinomas. Shown are two histological images of sections of a PyMT adenoma (left) and a PyMT carcinoma (right) stained for hematoxylin (purple) and smooth muscle actin (brown). Note that in the adenoma the smooth muscle actin-positive basement membrane is still present, but is lacking in the carcinoma. Moreover, tumor cells in the carcinoma invade the surrounding stroma (marked by the asterisk). Scale bars, 50 μm.
sc-12-0811_sm_SupplFigure2.pdf120KSupporting Information Figure S2. PyMT tumor growth was not detectable affected by the induction of the confetti randomizer using tamoxifen. (A): Shown are histological images of sections of a PyMT tumor treated or not treated with tamoxifen stained for hematoxylin and eosin. Scale bars, 50 μm. (B): Other PyMT sections of the same tumors as in A were stained with an anti-Ki67 antibody, and the number of proliferative cells per imaging field (i.f.) was analyzed (N=25). The average number and standard error of the mean of proliferative Ki67+ cells were plotted. (C): Other sections were stained for apoptotic cells with an anti cleaved caspase-3 antibody. The average number and standard error of the mean of apoptotic cells per imaging field (N=25) were plotted. Shown are the average number and standard error of the mean. (D): Growth of PyMT tumors in control (gray line) and tamoxifen-treated animals (red line) plotted over time. Shown are the average values and standard error of the mean for 4 mice.

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