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Cancer Stem Cells
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Brief Report: Intravital Imaging of Cancer Stem Cell Plasticity in Mammary Tumors†‡§
Article first published online: 25 FEB 2013
DOI: 10.1002/stem.1296
Copyright © 2012 AlphaMed Press
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How to Cite
Zomer, A., Ellenbroek, S. I. J., Ritsma, L., Beerling, E., Vrisekoop, N. and Van Rheenen, J. (2013), Brief Report: Intravital Imaging of Cancer Stem Cell Plasticity in Mammary Tumors. STEM CELLS, 31: 602–606. doi: 10.1002/stem.1296
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Author contributions: A.Z.: collection and assembly of the data, data analysis and interpretation, manuscript writing, and final approval of the manuscript; S.I.J.E. and N.V.: data analysis and interpretation, manuscript writing, and final approval of the manuscript; L.R.: developed the mammary imaging window, collection and assembly of the data, and final approval of the manuscript; E.B.: collection and assembly of the data and final approval of the manuscript; J.v.R.: conception and design, financial support, manuscript writing, and final approval of the manuscript.
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Disclosure of potential conflicts of interest is found at the end of this article.
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First published online in STEM CELLSEXPRESS December 7, 2012.
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Publication History
- Issue published online: 25 FEB 2013
- Article first published online: 25 FEB 2013
- Accepted manuscript online: 7 DEC 2012 05:35AM EST
- Manuscript Accepted: 15 NOV 2012
- Manuscript Received: 29 AUG 2012
Funded by
- VIDI. Grant Number: 91710330
- Dutch Organization of Scientific Research (NWO)
- Dutch Cancer Society
- KWF
- HUBR. Grant Number: 2009-4621
- Association for International Cancer Research (AICR, 13-0297). Grant Numbers: 175.010.2007.00, 834.11.002
- Dutch Organization of Scientific Research (NWO)
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Additional Supporting Information may be found in the online version of this article.
| Filename | Format | Size | Description |
|---|---|---|---|
| sc-12-0811_sm_SupplFigure1.pdf | 195K | Supporting Information Figure S1. PyMT expression induced the formation of adenomas that progressed to carcinomas. Shown are two histological images of sections of a PyMT adenoma (left) and a PyMT carcinoma (right) stained for hematoxylin (purple) and smooth muscle actin (brown). Note that in the adenoma the smooth muscle actin-positive basement membrane is still present, but is lacking in the carcinoma. Moreover, tumor cells in the carcinoma invade the surrounding stroma (marked by the asterisk). Scale bars, 50 μm. | |
| sc-12-0811_sm_SupplFigure2.pdf | 120K | Supporting Information Figure S2. PyMT tumor growth was not detectable affected by the induction of the confetti randomizer using tamoxifen. (A): Shown are histological images of sections of a PyMT tumor treated or not treated with tamoxifen stained for hematoxylin and eosin. Scale bars, 50 μm. (B): Other PyMT sections of the same tumors as in A were stained with an anti-Ki67 antibody, and the number of proliferative cells per imaging field (i.f.) was analyzed (N=25). The average number and standard error of the mean of proliferative Ki67+ cells were plotted. (C): Other sections were stained for apoptotic cells with an anti cleaved caspase-3 antibody. The average number and standard error of the mean of apoptotic cells per imaging field (N=25) were plotted. Shown are the average number and standard error of the mean. (D): Growth of PyMT tumors in control (gray line) and tamoxifen-treated animals (red line) plotted over time. Shown are the average values and standard error of the mean for 4 mice. |
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