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Additional Supporting Information may be found in the online version of this article.

FilenameFormatSizeDescription
sc-12-0514_sm_SupplFigure1.tif518KFigure S1. Effect of TGF-β1 on the growth of MNNG bulk cells in serum-free DF medium supplemented with 1% FBS.
sc-12-0514_sm_SupplFigure2.tif532KFigure S2. Effect of SB431542 on the growth of MNNG bulk cells in serum-free DF medium supplemented with 1% FBS.
sc-12-0514_sm_SupplFigure3.tif2542KFigure S3. Representative image of residual adherent cells cultured in 3D matrigel supplemented with VEGF-A for 8 days and 21 days. Scale bar, 100μm.
sc-12-0514_sm_SupplFigure4.tif916KFigure S4. GO Biological process analysis of differentially regulated genes in OSCs, iOSCs and hypoxia induced sarcospheres.
sc-12-0514_sm_SupplTable1.pdf110KTable S1. Gene set enrichment analysis of gene expression. *Numbers indicate that analyses use part of GSE33382 data from GSM825626 to GSM825709 except data which histopathological grade are unknown. - Numbers indicate that analyses use part of GSE33382 data from GSM825631 to GSM825709 except data which histopathological grade are unknown. For the decision of chemotherapeutic treatment huvos grade 1 and 2 were considered as poor responders and huvos grade 3 and 4 as good responders [54]. #Numbers indicate that analyses use part of GSE19276 data from GSM481071 to GSM481114 except data which chemotherapy response are unknown.
sc-12-0514_sm_SupplTable2.pdf98KTable S2. Selected transcripts regulated by TGF-β1.
sc-12-0514_sm_SupplTable3.pdf169KTable S3. Antibodies used in this study.
sc-12-0514_sm_SupplTable4.pdf89KTable S4. Primers used in this study.
sc-12-0514_sm_SupplTable5.pdf118KTable S5. Gene sets used in gene set enrichment analysis.

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