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Tissue-Specific Stem Cells
Article first published online: 25 FEB 2013
Copyright © 2012 AlphaMed Press
Volume 31, Issue 3, pages 526–535, March 2013
How to Cite
Deschepper, M., Manassero, M., Oudina, K., Paquet, J., Monfoulet, L.-E., Bensidhoum, M., Logeart-Avramoglou, D. and Petite, H. (2013), Proangiogenic and Prosurvival Functions of Glucose in Human Mesenchymal Stem Cells upon Transplantation. STEM CELLS, 31: 526–535. doi: 10.1002/stem.1299
Author contributions: M.D.: conception and design, collection and/or assembly of data, data analysis and interpretation, and manuscript writing; M.M. and K.O.: collection and/or assembly of data; J.P. and L.E.M.: conception and design and data analysis and interpretation; M.B.: provision of study material or patients; D.L-A.: data analysis and interpretation and financial support; H.P.: conception and design, data analysis and interpretation, financial support, manuscript writing, and final approval of manuscript.
Disclosure of potential conflicts of interest is found at the end of this article.
First published online in STEM CELLSEXPRESS December 7, 2012.
- Issue published online: 25 FEB 2013
- Article first published online: 25 FEB 2013
- Accepted manuscript online: 7 DEC 2012 05:40AM EST
- Manuscript Accepted: 14 NOV 2012
- Manuscript Revised: 8 NOV 2012
- Manuscript Received: 23 JUL 2012
- Fonds d'amorçage Biothérapie. Grant Numbers: BTH06003, ANR 07-RIB-011-01 MYOCELLOS, ANR 08-TECS-004 GLASSBONE
- Contrat d'Interface AP-HP/INSERM
- Human mesenchymal stem cells;
- Marrow stromal cells;
A major limitation in the development of cellular therapies using human mesenchymal stem cells (hMSCs) is cell survival post-transplantation. In this study, we challenged the current paradigm of hMSC survival, which assigned a pivotal role to oxygen, by testing the hypothesis that exogenous glucose may be key to hMSC survival. We demonstrated that hMSCs could endure sustained near-anoxia conditions only in the presence of glucose. In this in vitro cell model, the protein expressions of Hif-1α and angiogenic factors were upregulated by the presence of glucose. Ectopically implanted tissue constructs supplemented with glucose exhibited four- to fivefold higher viability and were more vascularized compared to those without glucose at day 14. These findings provided the first direct in vitro and in vivo demonstration of the proangiogenic and prosurvival functions of glucose in hMSC upon transplantation and identified glucose as an essential component of the ideal scaffold for transplanting stem cells. STEM CELLS2013;31:526–535