Identification of Nonepithelial Multipotent Cells in the Embryonic Olfactory Mucosa

Authors

  • Mercedes Tomé,

    1. Division of Clinical Neuroscience, Glasgow Biomedical Research Centre, University of Glasgow, Glasgow, Scotland
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  • Susan L. Lindsay,

    1. Division of Clinical Neuroscience, Glasgow Biomedical Research Centre, University of Glasgow, Glasgow, Scotland
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  • John S. Riddell,

    1. Division of Neuroscience and Biomedical Systems, Institute of Biomedical and Life Sciences, University of Glasgow, Glasgow, Scotland
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  • Susan C. Barnett

    Corresponding author
    1. Division of Clinical Neuroscience, Glasgow Biomedical Research Centre, University of Glasgow, Glasgow, Scotland
    • Division of Clinical Neuroscience, Biomedical Research Centre, Room B417, 120 University Place, University of Glasgow, Glasgow G12 8TA, Scotland
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    • Telephone: (0)141 330 8409/4353


  • Author contributions: M.T., J.S.R., and S.C.B.: contributed to manuscript writing; M.T. and S.C.B.: designed experiments; J.S.R. and S.C.B.: obtained financial support; M.T.: collected and assembled data; S.L.L.: carried out human tissue staining and fluorescence-activated cell sorting analysis; S.C.B.: approved manuscript.

  • First published online in STEM CELLS EXPRESS May 21, 2009.

Abstract

Olfactory mucosal (OM) tissue, a potential source of stem cells, is currently being assessed in the clinic as a candidate tissue for transplant-mediated repair of spinal cord injury. We examined the ability of embryonic rat OM tissue to generate stem cells using culture conditions known to promote neural stem cell proliferation. Primary spheres formed that proliferated and exhibited two main morphologies: (a) CNS neurosphere-like (OM-I) and (b) small, tight spheroid-like (OM-II). The OM-I spheres expressed the neural stem cell marker nestin but also markers of peripheral glia, neurons, and connective tissue. Further studies demonstrated the presence of multipotential mesenchymal-like stem cells within OM-I spheres that differentiated into bone, adipose, and smooth muscle cells. In contrast, the OM-II spheres contained mainly cytokeratin-expressing cells. Immunolabeling of rat olfactory tissue with Stro-1, CD90, and CD105 showed the presence of multipotent mesenchymal cells in the lamina propria, whereas cytokeratin was expressed by the epithelial cells of the olfactory epithelium. In addition, a comparable pattern of immunoreactivity was detected in human tissue using Stro-1 and cytokeratin, suggesting the presence of similar cells in this tissue. The identification of a nonepithelial multipotent cell in the OM may explain the varied reports on olfactory stem cell differentiation capacity in vitro and in vivo and illustrates the cellular complexity of this tissue as a potential source of stem cells for transplantation and translation to the clinic. STEM CELLS 2009;27:2196–2208

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