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Tissue-Specific Stem Cells
Version of Record online: 25 FEB 2013
Copyright © 2012 AlphaMed Press
Volume 31, Issue 3, pages 536–546, March 2013
How to Cite
Katagiri, T., Kawamoto, H., Nakakuki, T., Ishiyama, K., Okada-Hatakeyama, M., Ohtake, S., Seiki, Y., Hosokawa, K. and Nakao, S. (2013), Individual Hematopoietic Stem Cells in Human Bone Marrow of Patients with Aplastic Anemia or Myelodysplastic Syndrome Stably Give Rise to Limited Cell Lineages. STEM CELLS, 31: 536–546. doi: 10.1002/stem.1301
Author contributions: H.K.: developed the concept of the study and supervised the project, designed the experiments, wrote the paper, and approved the final version of this paper; S.N.: developed the concept of the study and supervised the project, wrote the paper, and approved the final version of this paper; T.K. and T.N.: designed the experiments, performed the experiments and analyzed the data, wrote the paper, and approved the final version of this paper; K.I. and M.O.-H.: designed the experiments and approved the final version of this paper; S.O., Y.S. and K.H. :performed the experiments and analyzed the data, approved the final version of this paper. T.K., H.K., and T.N. contributed equally to this article.
Disclosure of potential conflicts of interest is found at the end of this article.
First published online in STEM CELLSEXPRESS January 12, 2013.
- Issue online: 25 FEB 2013
- Version of Record online: 25 FEB 2013
- Accepted manuscript online: 12 JAN 2013 07:10AM EST
- Manuscript Accepted: 26 NOV 2012
- Manuscript Revised: 12 NOV 2012
- Manuscript Received: 21 SEP 2012
- Ministry of Education, Culture, Sports, Science and Technology of Japan. Grant Number: KAKENHI No. 21390291
- Ministry of Health, Labor and Welfare of Japan
Additional Supporting Information may be found in the online version of this article.
|sc-12-0887_sm_supplFigure1.tif||2795K||Supplement Figure 1. Illustration of a cut end plane of a sphere that is formed by clones of an HSC in bone marrow and specification of simulation area. A. Black and white ellipsoids denote clones and HSC on the plane, respectively. B. Two-dimensional simulation area (30mm×30mm) contains several commitment planes (grayed circles). An enlarged view of the simulation area is shown on the right side, in which each cell (filled circle) is located on a coordinate grid.|
|sc-12-0887_sm_supplFigure2.pdf||944K||Supplement Figure 2. Specification of mosaic-like hematopoietic environment. A. AM, AE, AB and AT denote size of their commitment areas of M, E, B and T, respectively. B. Total AM, Total AE, Total AB and Total AT denote summation of all commitment areas of M, E, B and T in whole simulation area, respectively. C. Illustration of variable mosaic-like patterns generated by changing area ratio (AR), total area ratio (TAR) and scaling factor (SF). Twelve patterns out of 112 are shown. 17|
|sc-12-0887_sm_supplFigure3a.pdf||1296K||Supplement Figure 3. Histograms regarding all 112 mosaic patterns. The horizontal and vertical axes denote clone pattern (METB, MET, MEB, MTB, ETB, ME, MT, MB, ET, EB, TB, M, E, B and T starting from the left) and count. The title specifies a combination of area ratio (AR), scaling factor (SF) and total area ratio (TAR), and the parenthetic value denotes sum of squared differences (SSD). A histogram with the SSD less than 0.5 has a red-colored title, and in addition if it contains a set of clone types that was found experimentally, it is highlighted with red-colored background.|
|sc-12-0887_sm_supplFigure3b.pdf||1305K||Supplement Figure 3b|
|sc-12-0887_sm_supplFigure4.pdf||1070K||Supplement Figure 4. The selected 17 mosaic patterns. The title specifies a combination of AR, SF and TAR as well as Simulation-method Figure 3.|
|sc-12-0887_sm_supplFigure5.pdf||784K||Supplement Figure 5. Initial HSC positions on Supplementary Movies.|
|sc-12-0887_sm_supplTable1.tif||104K||Supplement Table 1|
|sc-12-0887_sm_supplTable2.tif||135K||Supplement Table 2|
|sc-12-0887_sm_supplTable3.tif||183K||Supplement Table 3|
|sc-12-0887_sm_supplMovie.pdf||32K||Supplement Movie Legends|
|sc-12-0887_sm_supplMovie_1.mov||416K||Movie S1. Results as described in the supplementary note_Supplementary_Movie_1_for lineage type of METB.|
|sc-12-0887_sm_supplMovie_2.mov||467K||Movie S2. Results as described in the supplementary note_Supplementary_Movie_2_for lineage type of MET.|
|sc-12-0887_sm_supplMovie_3.mov||482K||Movie S3. Results as described in the supplementary note_Supplementary_Movie_3_for lineage type of MEB.|
|sc-12-0887_sm_supplMovie_4.mov||420K||Movie S4. Results as described in the supplementary note_Supplementary_Movie_4_for lineage type of ME.|
|sc-12-0887_sm_supplMovie_5.mov||344K||Movie S5. Results as described in the supplementary note_Supplementary_Movie_5_for lineage type of M.|
|sc-12-0887_sm_SupplementaryFigureLegends.pdf||11K||Supplement Figure Legends|
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