Q.W. and A.W.B. provided equal contributions.
Embryonic Stem Cells
Article first published online: 21 MAY 2009
Copyright © 2009 AlphaMed Press
Volume 27, Issue 11, pages 2637–2645, November 2009
How to Cite
Wu, Q., Bruce, A. W., Jedrusik, A., Ellis, P. D., Andrews, R. M., Langford, C. F., Glover, D. M. and Zernicka-Goetz, M. (2009), CARM1 Is Required in Embryonic Stem Cells to Maintain Pluripotency and Resist Differentiation. STEM CELLS, 27: 2637–2645. doi: 10.1002/stem.131
Disclosure of potential conflicts of interest is found at the end of this article.
First published online in STEM CELLS EXPRESS May 21, 2009; available online without subscription throught the open access option.
- Issue published online: 6 NOV 2009
- Article first published online: 21 MAY 2009
- Accepted manuscript online: 21 MAY 2009 12:00AM EST
- Manuscript Accepted: 30 APR 2009
- Manuscript Received: 29 OCT 2008
- 1Four-cell stage mouse blastomeres have different developmental properties. Development 2005; 132: 479–490., , et al.
- 2Histone arginine methylation regulates pluripotency in the early mouse embryo. Nature 2007; 445: 214–218., , et al.
- 3How is pluripotency determined and maintained? Development 2007; 134: 635–646..
- 4The homeoprotein Nanog is required for maintenance of pluripotency in mouse epiblast and ES cells. Cell 2003; 113: 631–642., , et al.
- 5Functional expression cloning of Nanog, a pluripotency sustaining factor in embryonic stem cells. Cell 2003; 113: 643–655., , et al.
- 6Interaction between Oct3/4 and Cdx2 determines trophectoderm differentiation. Cell 2005; 123: 917–929., , et al.
- 7Multipotent cell lineages in early mouse development depend on SOX2 function. Genes Dev 2003; 17: 126–140., , et al.
- 8Dissecting self-renewal in stem cells with RNA interference. Nature 2006; 442: 533–538., , et al.
- 9Pluripotency governed by Sox2 via regulation of Oct3/4 expression in mouse embryonic stem cells. Nat Cell Biol 2007; 9: 625–635., , et al.
- 10Hyperdynamic plasticity of chromatin proteins in pluripotent embryonic stem cells. Dev Cell 2006; 10: 105–116., , et al.
- 11Chromatin modifications and their function. Cell 2007; 128: 693–705..
- 12Chromatin signatures of pluripotent cell lines. Nat Cell Biol 2006; 8: 532–538., , et al.
- 13A bivalent chromatin structure marks key developmental genes in embryonic stem cells. Cell 2006; 125: 315–326., , et al.
- 14Polycomb complexes repress developmental regulators in murine embryonic stem cells. Nature 2006; 441: 349–353., , et al.
- 15The Oct4 and Nanog transcription network regulates pluripotency in mouse embryonic stem cells. Nat Genet 2006; 38: 431–440., , et al.
- 16The polycomb-group gene Ezh2 is required for early mouse development. Mol Cell Biol 2001; 21: 4330–4336., , et al.
- 17Control of developmental regulators by polycomb in human embryonic stem cells. Cell 2006; 125: 301–313., , et al.
- 18Normalization for cDNA microarray data: a robust composite method addressing single and multiple slide systematic variation. Nucleic Acids Res 2002; 30: e15., , et al.
- 19Linear models and empirical Bayes methods for assessing differential expression in microarray experiments. Stat Appl Genet Mol Biol 2004; 3:Article3..
- 20Controlling the False Discovery Rate: a practical and powerful approach to multiple testing. J Royal Stat Soc Ser B 1995; 57: 289–300., .
- 21Jmjd1a and Jmjd2c histone H3 Lys 9 demethylases regulate self-renewal in embryonic stem cells. Genes Dev 2007; 21: 2545–2557., , et al.
- 22Cluster analysis and display of genome-wide expression patterns. Proc Natl Acad Sci U S A 1998; 95: 14863–14868., , et al.
- 23GO::TermFinder—open source software for accessing Gene Ontology information and finding significantly enriched Gene Ontology terms associated with a list of genes. Bioinformatics 2004; 20: 3710–3715., , et al.
- 24Oct-3/4 and Sox2 regulate Oct-3/4 gene in embryonic stem cells. J Biol Chem 2005; 280: 5307–5317., , et al.
- 25Reciprocal transcriptional regulation of Pou5f1 and Sox2 via the Oct4/Sox2 complex in embryonic stem cells. Mol Cell Biol 2005; 25: 6031–6046., , et al.
- 26Octamer and Sox elements are required for transcriptional cis regulation of Nanog gene expression. Mol Cell Biol 2005; 25: 2475–2485., , et al.
- 27Transcriptional regulation of Nanog by OCT4 and SOX2. J Biol Chem 2005; 280: 24731–24737., , et al.
- 28Global gene expression profiling reveals similarities and differences among mouse pluripotent stem cells of different origins and strains. Dev Biol 2007; 307: 446–459., , et al.
- 29Arginine methylation at a glance. J Cell Sci 2007; 120: 4243–4246..
- 30Nanog safeguards pluripotency and mediates germline development. Nature 2007; 450: 1230–1234., , et al.
- 31Specific protein methylation defects and gene expression perturbations in coactivator-associated arginine methyltransferase 1-deficient mice. Proc Natl Acad Sci U S A 2003; 100: 6464–6468., , et al.
- 32Regulation of coactivator complex assembly and function by protein arginine methylation and demethylimination. Proc Natl Acad Sci U S A 2005; 102: 3611–3616., , et al.
- 33The activity and stability of the transcriptional coactivator p/CIP/SRC-3 are regulated by CARM1-dependent methylation. Mol Cell Biol 2007; 27: 120–134., , et al.
- 34Signaling within a coactivator complex: methylation of SRC-3/AIB1 is a molecular switch for complex disassembly. Mol Cell Biol 2006; 26: 7846–7857., , et al.
- 35The arginine methyltransferase CARM1 regulates the coupling of transcription and mRNA processing. Mol Cell 2007; 25: 71–83., , et al.
- 36Lipopolysaccharide-induced methylation of HuR, an mRNA-stabilizing protein, by CARM1. Coactivator-associated arginine methyltransferase J Biol Chem 2002; 277: 44623–44630., , et al.
- 37Coactivator-associated arginine methyltransferase 1, CARM1, affects pre-mRNA splicing in an isoform-specific manner. J Biol Chem 2005; 280: 28927–28935., , et al.