Telephone: 86-10-80728967; Fax: 86-10-80727535
Embryonic Stem Cells/Induced Pluripotent Stem Cells
Article first published online: 24 MAR 2013
Copyright © 2013 AlphaMed Press
Volume 31, Issue 4, pages 729–740, April 2013
How to Cite
Jiao, J., Dang, Y., Yang, Y., Gao, R., Zhang, Y., Kou, Z., Sun, X.-F. and Gao, S. (2013), Promoting Reprogramming by FGF2 Reveals that the Extracellular Matrix Is a Barrier for Reprogramming Fibroblasts to Pluripotency. STEM CELLS, 31: 729–740. doi: 10.1002/stem.1318
Author contributions: J.J.: conception and design, collection and/or assembly of data, data analysis and interpretation, and manuscript writing; Y.D., Y.Y., R.G., Y.Z., Z.K., and X.S.: provision of study material and data analysis and interpretation; S.G.: conception and design, financial support, data analysis and interpretation, manuscript writing, and final approval of manuscript.
Disclosure of potential conflicts of interest is found at the end of this article.
First published online in STEM CELLS EXPRESS January 10, 2013.
- Issue published online: 24 MAR 2013
- Article first published online: 24 MAR 2013
- Manuscript Accepted: 12 DEC 2012
- Manuscript Received: 3 JUL 2012
- Ministry of Science and Technology. Grant Numbers: 2010CB944900, 2011CB812700, 2011CB964800
- Fibroblast growth factor 2;
- Induced pluripotent stem cells;
- Extracellular matrix
Leukemia inhibitory factor and bone morphogenetic protein signaling pathways play important roles in maintaining the self-renewal of mouse embryonic stem cells (ESCs). In contrast, the supplementation of fibroblast growth factor 2 (FGF2) in culture promotes mouse ESC differentiation. It has been proposed that factors that are adverse for maintaining the self-renewal of ESCs might play detrimental roles in the transcription factor-mediated reprogramming of somatic cells to pluripotency. However, recent evidence has revealed that reprogramming efficiency could be improved by FGF2, while the underlying molecular mechanism remains unknown. In this study, we dissected the roles of FGF2 in promoting mouse fibroblast reprogramming and disclosed the molecular mechanism behind this process. We used both primary induction and secondary inducible reprogramming systems and demonstrated that supplementation with FGF2 in the early phase of induced pluripotent stem cell induction could significantly increase reprogramming efficiency. Moreover, we discovered that many extracellular matrix candidate genes were significantly downregulated in fibroblasts treated with FGF2, and in particular, the synthesis of collagen could be greatly reduced by FGF2 treatment. Subsequently, we demonstrated that collagen is a barrier for reprogramming fibroblast cells to pluripotency, and the decreasing of collagen either by collagenase treatment or downregulation of collagen gene expression could significantly improve the reprogramming efficiency. Our results reveal a novel role of the extracellular matrix in mediating fibroblasts reprogramming. STEM CELLS 2013;31:729–740