Author contributions: Y.H., L.L., X.Y., G.W., X.P., H.Z., and C.F.: collection and/or assembly of data; J.Z.: provision of study material; S.Y.: manuscript writing and/or provision of study materials; L.Z.: provision of study materials and/or financial support; Q.X.: conception and design, financial support, administrative support, provision of study material, collection and/or assembly of data, data analysis and interpretation, manuscript writing, and final approval of manuscript. Y.H. and L.L. contributed equally to this article.
Embryonic Stem Cells/induced Pluripotent Stem Cells
Article first published online: 24 APR 2013
Copyright © 2013 AlphaMed Press
Volume 31, Issue 5, pages 906–917, May 2013
How to Cite
Huang, Y., Lin, L., Yu, X., Wen, G., Pu, X., Zhao, H., Fang, C., Zhu, J., Ye, S., Zhang, L. and Xiao, Q. (2013), Functional Involvements of Heterogeneous Nuclear Ribonucleoprotein A1 in Smooth Muscle Differentiation from Stem Cells In Vitro and In Vivo. STEM CELLS, 31: 906–917. doi: 10.1002/stem.1324
Disclosure of potential conflicts of interest is found at the end of this article.
First published online in STEM CELLSEXPRESS January 17, 2013.
- Issue published online: 24 APR 2013
- Article first published online: 24 APR 2013
- Accepted manuscript online: 17 JAN 2013 10:47PM EST
- Manuscript Accepted: 14 DEC 2012
- Manuscript Received: 10 SEP 2012
- British Heart Foundation. Grant Numbers: FS/09/044/28007, PG/11/40/28891
- National Natural Science Foundation of China. Grant Numbers: 81270001, 81270180
- British Heart Foundation Intermediate Basic Science Research Fellowship. Grant Number: FS/09/044/28007
- British Heart Foundation. Grant Number: PG/11/40/28891
- London Cardiovascular Biomedical Research Unit
- National Institute of Health Research
Additional Supporting Information may be found in the online version of this article.
|sc-12-0845_sm_SupplTabe1.pdf||18K||Supplementary Table 1|
|sc-12-0845_sm_SupplFigure1.TIF||1588K||Figure S1. hnRNPA1 is specific for SMC differentiation from ES cells on collagen IV. ES cells cultured on collagen IV were transfected with pCMV-hnRNPA1 or empty vector control pCMV5 (1μg/106 cells) (A), or random control siRNA or hnRNPA1 siRNA (B), respectively. The cells were harvested and subjected to real-time RT-PCR analysis with sets of primers specific for different cell lineages genes: Flt-1 and CD144 for endothelial, Ddr2 and Thy1 for cardiac fibroblast, Tnnc 1 and actc1 for cardiac myocytes, Alcam and CD133 for hematopoietic progenitor, Dlx3 and Tpbg for trophoblast, CD29 and CD44 for mesenchymal, nestin and Gap43 for neural. The data are means±S.E.M of three independent experiments.|
|sc-12-0845_sm_SupplFigure2.TIF||1911K||Figure S2. Knockdown hnRNPA1 reduces calponin-positive SMCs derived from stem cells in vivo. Frozen sections from implants were subjected to double immunofluorescence staining with antibodies against beta-galactosidase (β-gal) and SMC marker calponin. The total numbers of Calponin-positive cells per field were counted by two well-trained independent investigators blinded to the treatments, from four random high power fields (200x) in each section, two sections from each implant and five implants for each group. Representative images (top panels) and quantitative data (bar graphs) were presented here. *p< 0.05.|
|sc-12-0845_sm_SupplFigure3.TIF||1366K||Figure S3 Schematic illustration of Acta2 (A) and Tagln (B) gene promoter regions.|
|sc-12-0845_sm_SupplFigure4.TIF||1538K||Figure S4 Schematic illustration of SRF (A), MEF2c (B) and Myocd (C) gene promoter regions. The number within the promoter region was defined according to its position with the start condon (‘A’: +1) of respective genes. Black region in respective gene represents the promoter we used in this study.|
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