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Embryonic Stem Cells/induced Pluripotent Stem Cells
Article first published online: 24 APR 2013
Copyright © 2013 AlphaMed Press
Volume 31, Issue 5, pages 918–927, May 2013
How to Cite
Chang, K.-H. and Li, M. (2013), Clonal Isolation of an Intermediate Pluripotent Stem Cell State. STEM CELLS, 31: 918–927. doi: 10.1002/stem.1330
Author contributions: K.H.C.: concept and design, execution of all experiments, data analysis and interpretation, obtained funding, and wrote the paper; M.L.: concept and design, data analysis and interpretation, obtained funding, and wrote and finalized the paper.
Disclosure of potential conflicts of interest is found at the end of this article.
First published online in STEM CELLSEXPRESS January 22, 2013.
- Issue published online: 24 APR 2013
- Article first published online: 24 APR 2013
- Accepted manuscript online: 22 JAN 2013 08:53AM EST
- Manuscript Accepted: 13 DEC 2012
- Manuscript Received: 30 JUL 2012
- Chang Gung Memorial Hospital, Taipei, Taiwan. Grant Number: CMRPG 360041-43 and 3A0691-93
- UK Medical Research Council. Grant Number: G117/560 and U120005004
- National Science Council, Executive Yuan, Taiwan. Grant Number: NSC 100-2314-B-182A-076-MY1-2
Additional Supporting Information may be found in the online version of this article.
|sc-12-0708_sm_SupplFigure1.tif||2964K||Figure S1. Colony-forming assay of IESC clones in LIF and activin. One thousand cells of each of the 34 clones were plated in LIF, activin, or medium alone for 5 days, followed by AP staining. The clones displaying similar responsiveness were grouped together. Clones 10 and 16, which were responsive to both LIF and activin conditions, were designated as IESC1 and IESC2.|
|sc-12-0708_sm_SupplFigure2.pdf||789K||Supporting information figure S2. Reversion of IESC state by LIF. (A) Heat map illustrating the expression level of 26 frequently referred pluripotency and early lineage gene markers, and (B) Hierarchy clustering of 41174 probes expressed by ESCs, IESC1s (passage 15), IESC1-Ls (passage 3) and Epi-IESC1s (passage 10). High expression level is indicated in shades of red and low expression, in shades of green.|
|sc-12-0708_sm_SupplFigure3.pdf||697K||Supporting information figure S3. Delayed development of IESC-derived chimeric embryos. Blastocyst embryos were injected with IESC1-GFP and embryos harvested at 9.5dpc. (A) Shown is a developmental delayed chimeric embryo with high IESC contributon. (B) Low level of IESC contribution in normal looking 9.5 dpc embryo. (C) No chimera was obtained in the 38 pups recovered at birth. Scale bars, 400μm.|
|sc-12-0708_sm_SupplTable1.pdf||60K||Supplementary Table 1|
|sc-12-0708_sm_SupplTable2.pdf||25K||Supplementary Table 2|
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