Additional Supporting Information may be found in the online version of this article.

sc-12-0708_sm_SupplFigure1.tif2964KFigure S1. Colony-forming assay of IESC clones in LIF and activin. One thousand cells of each of the 34 clones were plated in LIF, activin, or medium alone for 5 days, followed by AP staining. The clones displaying similar responsiveness were grouped together. Clones 10 and 16, which were responsive to both LIF and activin conditions, were designated as IESC1 and IESC2.
sc-12-0708_sm_SupplFigure2.pdf789KSupporting information figure S2. Reversion of IESC state by LIF. (A) Heat map illustrating the expression level of 26 frequently referred pluripotency and early lineage gene markers, and (B) Hierarchy clustering of 41174 probes expressed by ESCs, IESC1s (passage 15), IESC1-Ls (passage 3) and Epi-IESC1s (passage 10). High expression level is indicated in shades of red and low expression, in shades of green.
sc-12-0708_sm_SupplFigure3.pdf697KSupporting information figure S3. Delayed development of IESC-derived chimeric embryos. Blastocyst embryos were injected with IESC1-GFP and embryos harvested at 9.5dpc. (A) Shown is a developmental delayed chimeric embryo with high IESC contributon. (B) Low level of IESC contribution in normal looking 9.5 dpc embryo. (C) No chimera was obtained in the 38 pups recovered at birth. Scale bars, 400μm.
sc-12-0708_sm_SupplTable1.pdf60KSupplementary Table 1
sc-12-0708_sm_SupplTable2.pdf25KSupplementary Table 2

Please note: Wiley Blackwell is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.