• Open Access

Concise Review: Maturation Phases of Human Pluripotent Stem Cell-Derived Cardiomyocytes§

Authors

  • Claire Robertson,

    1. Department of Biomedical Engineering, University of California, Irvine, Irvine, California, USA
    2. Edwards Lifesciences Center for Advanced Cardiovascular Technology, University of California, Irvine, Irvine, California, USA
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  • David D. Tran,

    1. Edwards Lifesciences Center for Advanced Cardiovascular Technology, University of California, Irvine, Irvine, California, USA
    2. Department of Chemical Engineering and Materials Science, University of California, Irvine, Irvine, California, USA
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  • Steven C. George

    Corresponding author
    1. Department of Biomedical Engineering, University of California, Irvine, Irvine, California, USA
    2. Edwards Lifesciences Center for Advanced Cardiovascular Technology, University of California, Irvine, Irvine, California, USA
    3. Department of Chemical Engineering and Materials Science, University of California, Irvine, Irvine, California, USA
    4. Department of Medicine, University of California, Irvine, Irvine, California, USA
    • Edwards Lifesciences Professor, Director, Edwards Lifesciences Center for Advanced Cardiovascular Technology, Department of Biomedical Engineering, 2420 Engineering Hall, University of California, Irvine, Irvine, CA 92697-2730, USA
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    • Telephone: (949) 824-3941; Fax: (949) 824-9968


  • Author contributions: Claire Robertson, Conception and Design, Manuscript Writing; David Tran, Manuscript Writing, Graphic Design; Steven George, Financial Support, Final Approval of Manuscript.

  • Disclosure of potential conflicts of interest is found at the end of this article.

  • §

    First published online in STEM CELLSEXPRESS January 25, 2013.

Abstract

Human pluripotent stem cell-derived cardiomyocytes (hPS-CM) may offer a number of advantages over previous cardiac models, however, questions of their immaturity complicate their adoption as a new in vitro model. hPS-CM differ from adult cardiomyocytes with respect to structure, proliferation, metabolism and electrophysiology, better approximating fetal cardiomyocytes. Time in culture appears to significantly impact phenotype, leading to what can be referred to as early and late hPS-CM. This work surveys the phenotype of hPS-CM, including structure, bioenergetics, sensitivity to damage, gene expression, and electrophysiology, including action potential, ion channels, and intracellular calcium stores, while contrasting fetal and adult CM with hPS-CM at early and late time points after onset of differentiation. STEM CELLS 2013;31:829–837

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