• Open Access

Efficient Generation of Astrocytes from Human Pluripotent Stem Cells in Defined Conditions§

Authors


  • Author contributions: A.S.: collection and/or assembly of data, data analysis and interpretation, and manuscript writing; J.P. and Q.L.: collection and/or assembly of data; M.S.R.: conception and design and manuscript writing; X.Z.: conception and design, data analysis and interpretation, manuscript writing, and final approval of manuscript.

  • Disclosure of potential conflicts of interest is found at the end of this article.

  • §

    First published online in STEM CELLSEXPRESS January 22, 2013.

Abstract

Astrocytes can be generated from various tissue sources including human pluripotent stem cells (PSC). In this manuscript, we describe a chemically defined xeno-free medium culture system for rapidly generating astrocytes from neural stem cells derived from PSC. We show that astrocyte development in vitro, mimics normal development in vivo, and also passes through a CD44+ astrocyte precursor stage. Astrocytes generated by our method display similar gene expression patterns, morphological characteristics and functional properties to primary astrocytes, and they survive and integrate after xenotransplantation. Whole genome expression profiling of astrocyte differentiation was performed at several time points of differentiation, and the results indicate the importance of known regulators and identify potential novel regulators and stage-specific lineage markers. STEM CELLS 2013;31:941–952

Ancillary