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Additional Supporting Information may be found in the online version of this article.

FilenameFormatSizeDescription
sc-12-0909_sm_SupplFigure1.tif335KFigure S1. The efficiency of shRNA-mediated knockdown of TDH was confirmed in R1 ES cells by Western blot analysis (A), and by real-time RT-PCR analysis (B).
sc-12-0909_sm_SupplFigure2.tif238KFigure S2. Oct4-EGFP MEF cells were transduced with either OSCK or OSCK plus TDH. The cells were cultured in the indicated culture medium. 16 days after transduction, GFP-positive colonies were counted. Data were represented as mean±SD from three independent experiments. ns indicates no significant relationship.
sc-12-0909_sm_SupplFigure3.tif564KFigure S3. (A) Listed were the four miRNA candidates that could target to 3′UTR of TDH. (B) miR-9, miR-149, miR-218 and miR-31 were individually transfected into MEF cells together with the indicated pSICHECK2-based luciferase reporter construct. 24 h after transfection, reporter activity was measured and plotted after normalizing with respect to Renilla luciferase activity (mean±SD). * and ns indicate P<0.05 and no significance, respectively. (C) miR-9, miR-149, miR-218 and miR-31 mimics were individually transfected into R1 ES cells. 36 h after transfection, cell lysates were subjected to Western blot analysis with anti-TDH antibody. (D) The successful expression of miR-9, miR-149, miR-218 and miR-31 was confirmed by real-time RT-PCR analysis.
sc-12-0909_sm_SupplFigure4.tif232KFigure S4. miR-9 mimics or miR-9 inhibitors were transfected into MEF cells together with the indicated reporter construct as indicated. 24 h after transfection, reporter activity was measured and plotted after normalizing with respect to Renilla luciferase activity (mean±SD). *** indicates P<0.001.
sc-12-0909_sm_SupplFigure5.pdf959KFigure S5. Structural determinants of the TDH-PRMT5 interaction. (A) Schematic representation of PRMT5 and its mutants used in this study. (B) Wild type PRMT5 and the indicated PRMT5 mutants were individually transfected into HEK 293T cells together with GFP-TDH. 24 h after transfection, cell lysates were immunoprecipitated with anti-Flag antibody. (C) Schematic representation of PRMT5 and PRMT5_. (D) Flag-PRMT5 or Flag-PRMT5_ was transfected into HEK 293T cells together with GFP-TDH. 24 h after transfection, cell lysates were immunoprecipitated with anti-Flag antibody. (E) Schematic representation of TDH and its deletion mutants used in this study. (F) Wild type TDH and the indicated TDH mutants were individually transfected into HEK 293T cells together with Flag-PRMT5. 24 h after transfection, cell lysates were immunoprecipitated with anti-Flag antibody.
sc-12-0909_sm_SupplTable1.tif1089KSupplementary Table 1

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