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Tissue Specific Stem Cells
Article first published online: 24 APR 2013
Copyright © 2013 AlphaMed Press
Volume 31, Issue 5, pages 1001–1009, May 2013
How to Cite
Shinoda, G., De Soysa, T. Y., Seligson, M. T., Yabuuchi, A., Fujiwara, Y., Huang, P. Y., Hagan, J. P., Gregory, R. I., Moss, E. G. and Daley, G. Q. (2013), Lin28a Regulates Germ Cell Pool Size and Fertility. STEM CELLS, 31: 1001–1009. doi: 10.1002/stem.1343
Author contributions: G.S.: conception and design, collection and/or assembly of data, data analysis and interpretation, manuscript writing, and final approval of manuscript; T.Y.S. and M.T.S.: collection and/or assembly of data; A.Y., J.P.H., and R.I.G.: provision of study materials; Y.F. and P.Y.H.: collection and/or assembly of data, and data analysis and interpretation; E.G.M.: provision of study material or patients. G.Q.D.: conception and design, data analysis and interpretation, manuscript writing, and final approval of manuscript.
Disclosure of potential conflicts of interest is found at the end of this article.
First published online in STEM CELLSEXPRESS February 4, 2013.
- Issue published online: 24 APR 2013
- Article first published online: 24 APR 2013
- Accepted manuscript online: 4 FEB 2013 01:40AM EST
- Manuscript Accepted: 29 DEC 2012
- Manuscript Received: 20 AUG 2012
- Alex's Lemonade Stand Foundation
- Ellison Medical Foundation
- Hematology Center. Grant Number: P30 DK049216-17
- Mouse Embryonic Stem ESCs and Gene Targeting Core
- Eunice Kennedy Shriver NICHD/NIH (SCCPIR. Grant Number: U54-HD28934
- University of Virginia Center for Research in Reproduction Ligand Assay and Analysis Core
Additional Supporting Information may be found in the online version of this article.
|sc-12-0770_sm_SupplTable1.tiff||499K||Table S1 Results of in vitro fertilization using Lin28 +/+, +/−, and −/− sperm and wild type eggs. N=2-3. Numbers represent mean + SEM. Related to Fig. 5.|
|sc-12-0770_sm_SupplFigure1.tiff||1667K||Fig. S1. Targeted generation of a Lin28a null allele. (A) Genomic map of the Lin28a locus and Lin28a targeting construct. PCR primer positions and sequences for genotyping are shown. (B) Western blot showing Lin28a expression in Lin28a +/− and −/− ESCs. (C) PCR genotyping showing wild type (wt) and deleted (null) alleles. This strain (Strain 1) differs from the other strain we reported previously (Zhu et al., 2011; Strain 2) in that we used different vector backbones and the Neo cassette remains within the Lin28a allele of Strain 2. Related to Experimental procedures.|
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